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Indole Test Protocol

Downloaded from byIP: : Mon, 12 Aug 2019 20:04:30 American Society for Microbiology 2016 1 Indole Test Protocol | | Created: Tuesday, 08 December 2009 Author Maria P. MacWilliams Information History As far back as 1889, the Indole test was used as a means to distinguish between Escherichia coli andEnterobacter aerogenes (4). The numerous variations of the Indole test alone and in combination with other biochemical tests attest to the central role this test has played in the characterization of coliforms (gram-negative nonsporulating bacilli that ferment lactose, producing acid and gas) (5).

The chief requirement for culturing an organism prior to performing the indole test is that the medium contains a sufficient quantity of tryptophan (5). The presence of indole when a microbe is grown in a medium rich in tryptophan demonstrates that an …

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