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Indole Test Protocol

Downloaded from byIP: : Mon, 12 Aug 2019 20:04:30 American Society for Microbiology 2016 1 Indole Test Protocol | | Created: Tuesday, 08 December 2009 Author Maria P. MacWilliams Information History As far back as 1889, the Indole test was used as a means to distinguish between Escherichia coli andEnterobacter aerogenes (4). The numerous variations of the Indole test alone and in combination with other biochemical tests attest to the central role this test has played in the characterization of coliforms (gram-negative nonsporulating bacilli that ferment lactose, producing acid and gas) (5). The Indole test is still used as a classic test to distinguish Indole -positive E. coli from Indole -negativeEnterobacter and Klebsiella. (8) Purpose The Indole test screens for the ability of an organism to degrade the amino acid tryptophan and produce Indole . It is used as part of the IMViC procedures, a battery of tests designed to distinguish among members of the family Enterobacteriaceae.

Agar 2.0 g Bromcresol purple 0.02 g Bring to 1 liter with distilled water. Heat mixture to boiling to dissolve agar. Cool to 50°C. Dispense in 4.0 to 5.0 ml aliquots in 16-mm test tubes. Cap tubes and autoclave at 121°C under 15 psi pressure for 15 minutes. After autoclaving, allow tubes to cool in an upright position to form the agar deep.

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