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King's medium B Base - HiMedia Labs

Please refer disclaimer 's medium B BaseM1544 Kings medium B base is recommended for non-selective isolation, cultivation and pigment production of Pseudomonas speciesComposition**IngredientsGms / LitreProteose peptone hydrogen sulphate. pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams of dehydrated medium in 1000 ml distilled water containing 15 ml of glycerol. Heat to boiling to dissolvethe medium completely. Mix well. Sterilize by autoclaving at 15 lbs pressure (121 C) for 15 minutes. Aseptically pour intosterile Petri And InterpretationPseudomonas aeruginosa is known to produce two types of pigments, pyocyanin and fluorescein which is a characteristicproperty and aids in isolation of Pseudomonas from clinical material. An additional pigment called as pyorubin was reportedby king . Pyocyanin is green while fluorescein is fluorescent yellow and pyorubin is reddish brown. Some strains produce allthese pigments while the others produce one or two pigments.

Please refer disclaimer Overleaf. King's medium B Base M1544 Kings Medium B Base is recommended for non-selective isolation, cultivation and pigment production of Pseudomonas

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