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20. Principles and Applications of LC-MS/MS for the quantitative bioanalysis of Analytes in Various Biological Samples Ju-Seop Kang Department of Pharmacology & Clinical Pharmacology Laboratory, College of Medicine;. Division of Molecular Therapeutics Development, Hanyang Biomedical Research Institute, Hanyang University, Seoul, South Korea 1. Introduction Standard technology for analyte detection in clinical chemistry fields rely on indirect characteristics of an analyte , its absorption capability of light, chemical reactivity or physicochemical interaction with macro-molecules as like antigen-antibody interaction. Otherwise, analytes are detected directly from molecular characteristics as molecular mass and molecular disintegration patterns in mass spectrometric method.
Principles and Applications of LC-MS/MS for the Quantitative Bioanalysis of Analytes in Various Biological Samples 443 Fig. 1. Diagram of mass spectrometer system.
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