Transcription of Protocols and tips in protein purification
{{id}} {{{paragraph}}}
Example: air traffic controller
Protocols and tips in protein purification
Protocols and tips in protein purification or How to purify protein in one day Second edition 2018 Department of Molecular Biology & Biotechnology 2 3 Contents I. Introduction 7 II. General sequence of protein purification procedures Preparation of equipment and reagents Preparation and use of stock solutions Chromatography system Preparation of chromatographic columns Preparation of crude extract (cell free extract or soluble proteins fraction) Pre chromatographic steps Chromatographic steps Sequence of operations during IEC and HIC Ion exchange chromatography (IEC) Hydrophobic interaction chromatography (HIC) Gel filtration (SEC)
4.How to estimate purity of a protein preparation VII. Appendix 2 1. Prepare your solutions right! 2. Gel filtration Separation by molecule’s size Calibration plot Main reasons for “abnormal” mobility of some proteins during gel filtration 3. Time saving tips …
Loading..
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document: