Transcription of Real-time PCR handbook - Gene-Quantification
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Real-time PCR handbook - Gene-Quantification
Real-time PCR handbookThe image on this cover is of an OpenArray plate which is primarily used for mid-density Real-time PCR on the QuantStudio 12K Flex system. The figure above shows the commonly used formats for Real-time assays96- and 384-well plates384-well TaqMan Array cardsOpenArray plates162345 Basics of Real-time PCRD igital PCRE xperimental designPlate preparationData analysisTroubleshootingContentsBasics of Real-time Basics of Real-time PCR1 Introduction 3 Overview of Real-time PCR 4 Overview of Real-time PCR and Real-time PCR components 5 Real-time PCR analysis terminology 7 Real-time PCR fluorescence detection systems 11 Melting curve analysis 15 Use of passive reference dyes 16 Contamination prevention 17 Multiplex Real-time PCR 17 Internal controls and reference genes 19
Good primer design is one of the most important parameters in real-time PCR. This is why many researchers choose to purchase TaqMan ® Assay products—primers and probes for real-time PCR designed using a proven algorithm and trusted by scientists around the world. If you choose to design your own real-time PCR primers, keep in mind that
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