Transcription of Reticulocyte Count - Pearson Education
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Reticulocyte Count - Pearson Education
Reticulocyte CountPrincipleUsing a supravital stain ( , new methylene blue), residual ribosomal RNA within the reticulocytes is precipitated. An equal volume of stain is added to EDTA-anticoagulated blood, the dilution mixture incubated, and a smear is prepared. The smear is examined to determine the number of reticulocytes present. An erythrocyte containing two or more particles of blue-stained material is a Reticulocyte . The number of reticulocytes is expressed as a percentage of the total number of erythrocytes and Equipment1. New methylene blue N New methylene blue N g(certified by Biological Stain Commission)NaCl gQS with distilled water to 100 mLFilter staining solution through Whatman No. 1 filter paper prior to use2. Test tubes, 12 x 75 mm3. Pasteur pipets4. Glass slides5. Spreader slide6. Microscope7. Miller ocular disc8. Hand counterQuality ControlCommercial quality control materials with established control limits should be run periodically.
Reticulocyte Count Principle Using a supravital stain (e.g., new methylene blue), residual ribosomal RNA within the reticulocytes is precipitated.
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