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RIPA Buffer (10X) - Cell Signaling Technology

#9806 Store at 20 CRIPA Buffer (10X) 15 mlDescription: RIPA Buffer is used to lyse cells and RIPA Buffer : 20 mM Tris-HCl (pH ) 150 mM NaCl, 1 mM Na2 EDTA 1 mM EGTA 1% NP-40 1% sodium deoxycholate mM sodium pyrophosphate 1 mM b-glycerophosphate 1 mM Na3VO4 1 g/ml for Use1. If Buffer will be continually used, it is recommended that the 10x Buffer be kept at 4 C for 1-2 weeks. For longer periods of time, Buffer should be stored at -20 C. Aliquoting of 10x Buffer is recommended if many small experiments are to be Thaw 10x Buffer at 24-30 C, mixing Dilute 10X RIPA Buffer to a 1X solution using ddH2O.

1X RIPA Buffer: 20 mM Tris-HCl (pH 7.5) 150 mM NaCl, 1 mM Na 2 EDTA 1 mM EGTA 1% NP-40 1% sodium deoxycholate 2.5 mM sodium pyrophosphate 1 mM b-glycerophosphate 1 mM Na 3 VO 4 1 µg/ml leupeptin. Directions for Use 1. If buffer will be continually used, it is recommended that the 10x buffer be kept at 4°C for 1-2 weeks. For longer periods of ...

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