Transcription of Streptococcus Laboratory General Methods
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Streptococcus Laboratory General Methods
1 The reference used for compiling the Methods in Section I is: Murray, , Baron, E. J., Jorgensen, , Pfaller, , and Yolken, Manual of Clinical Microbiology, 8th ed. ASM Press: Washington, DC, 2003. The Streptococcus species identification Methods in Section II were compiled by Dr. Lynn Shewmaker. Also thanks to input from several individuals, including Richard Facklam and Lucia Teixeira. Section I. 1. Accuprobe-Enterococcus Accuprobe-Pneumococcus Test .. formation in carbohydrate solubility Test ..98. CAMP Catalase Clindamycin Esculin 1312. Gas from MRS Growth at 10 & 1917. Lancefield Group amino peptidase (LAP)..2119. Litmus Milk NaCl Tolerace 2624. Pyridoxal Requirement Test (Vitamin B6)..2725. Pyrrolidonlarylamindase (PYR).. Utilization Starch agar (extracellular polysaccharides).. 3230. Sucrose broth (5%).. 3331. Tellurite Vancomycin 3634. 37 Centers for Disease Control and PreventionUpdated March, 2014 2 Section II.
The blood agar plates used in the manner described above are for checking for purity of cultures. If any of the results are unusual or not expected or the culture is contaminated the test must be repeated. Vancomycin resistant streptococci or other unknowns other than leuconostocs and pediococci, which are intrinsically vancomycin resistant.
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