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SULFIDE-INDOLE-MOTILITY (SIM) TEST

Copyright 2019 by Chester R. Cooper, Jr. Microbiology Laboratory (BIOL 3702L) Page 1 of 5 SULFIDE-INDOLE-MOTILITY (SIM) TEST Principle and Purpose Using SULFIDE-INDOLE-MOTILITY (SIM) media, various Gram-negative enteric bacilli can be distinguished based upon their ability to release sulfide gas (H2S), produce indole from tryptophan, and move into the medium via the use of flagella. SIM medium contains ferrous ammonium sulfate and sodium thiosulfate. If a microbe hydrolyzes proteins rich in the amino acid cysteine, which contains a sulfur atom, the sulfur is released as H2S. The sulfide gas then combines with ferrous ammonium sulfate to form ferrous sulfide.

Removal of the needle otherwise may result in a false-positive interpretation. 4) Incubate all the tubes at 37°C for 18-24 hours. ... Sulfide-Indole-Motility (SIM) Test, Page 4 of 5 Interpretation of Results: A positive test for indole is indicated by the formation of a pink to red ... TABLE. Observations of Inoculated SIM Media (+ or ...

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