Transcription of Supporting Information - pnas.org
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Supporting Information - pnas.org
Supporting InformationPastorelli et al. Materials and endoscopic biopsies from males and females,aged 18 65 yr, and affected by CD or UC and noninflamed CONwere harvested from patients undergoingflexible sigmoidoscopyor colonoscopy for diagnostic or surveillance purposes at theUniversity of Virginia Digestive Health Center (UVA DHC) andwere used for total RNA and protein extraction. Similar gender-and age-matched colonic surgical specimens were obtained fromeither UC or CD patients, as well as noninflammatory CON, whowere admitted to the UVA DHC or the Cleveland Clinic Foun-dation (CCF) and underwent therapeutic bowel resection for in-flammatory disease or for malignant and other nonmalignantnoninflammatory conditions. Full-thickness colon tissues wereused for IHC (UVA) and as a source for mucosal IEC and LPMC(CCF).
RNeasy kit (Qiagen) and reverse-transcribed by using the Super-Script III kit (Invitrogen), both by manufacturers’ instructions. Preparation and Quantification of Total Protein Extracts.
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