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TransIT -LT1 Transfection Reagent - Mirus Bio

For Research Use Only. Technical Support Toll-free ( ) Direct: TransIT -LT1 Transfection Reagent Protocol for MIR 2300, 2304, 2305, 2306 Quick Reference Protocol, MSDS and Certificate of Analysis available at INTRODUCTION TransIT -LT1 Transfection Reagent is a broad spectrum Reagent that provides high efficiency plasmid DNA delivery in many mammalian cell types including primary cells . TransIT -LT1 is a low toxicity, serum-compatible Transfection Reagent that eliminates the need for any culture medium change. TransIT -LT1 is suitable for both transient and stable Transfection and can be used for many applications such as gene expression, viral production, shRNA expression and promoter analysis. SPECIFICATIONS MATERIALS Materials Supplied TransIT -LT1 Transfection Reagent is supplied in one of the following formats.

Transient plasmid DNA transfection protocol per well of a 6-well plate A. Plate cells 1. Approximately 18–24 hours before transfection, plate cells in 2.5 ml complete growth medium per well in a 6-well plate. Ideally cells should be ≥80% confluent prior to transfection. For adherent cells: Plate cells at a density of 0.8–3.0 × 105 cells/ml.

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  Transit, Transfection, Grantee, Cells, Transit lt1 transfection reagent

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