Cell Lysis Buffer (10X)
20 mM Tris-HCl (pH 7.5) 150 mM NaCl 1 mM Na2EDTA 1 mM EGTA 1% Triton 2.5 mM sodium pyrophosphate 1 mM b-glycerophosphate 1 mM Na 3 VO 4 1 µg/ml leupeptin Directions for use: 1. If buffer will be continually used, it is recommended that the 10x buffer be kept at 4°C for 1-2 weeks. For longer periods of time, buffer should be stored at –20°C.
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