Fundamentals of Protein Chemistry - Duke GCB
TCA Precipitation: (if [protein] is >0.05 mg/mL) Chill protein in a microcentrifuge tube to 0°C . Add 1/9 volume of cold 100% w/v TCA. Vortex . Incubate at 0°C for 30-60 min. Spin down in microcentrifuge. Remove supernatant. Wash pellet 3x w/ 200 μL cold acetone (do not vortex)
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