HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
9 2) Reverse phase chromatography - utilizes a: Uses reverse phase columns = contains nonpolar bonded phase s.p. Octyl = C8 Octadecyl = C18 nonpolar s.p. and polar m.p. Application: Separation of nonpolar compounds Example of nonpolar bonded (stationary) phases: Example of polar mobile phases Reverse phase chromatography (Cont.) Hydrocarbons (with C 8 and C 18; …
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