Ni-NTA Purification System
denatured lysate and then follow the hybrid protocol on page 20. Note that the hybrid protocol may not restore activity in all cases, and should be tested with your particular protein. 6. Remove 5 μL of the lysate for SDS-PAGE analysis. Store the remaining lysate on ice or freeze at –20°C. When ready to use, proceed to the protocol on page 16.
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