Ni-NTA Purification System

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Ni-NTA Purification System For Purification of polyhistidine-containing recombinant proteins Catalog Numbers K950-01, K953-01, R901-01, R901-10, R901-15. Document Part Number 25-0496. Publication Number MAN0000270. Revision For Research Use Only. Not for use in diagnostic procedures. 2. Contents Kit Contents and Storage ........................................ ........................................ ........................................ ............... 4 Accessory Products ........................................ ........................................ ........................................ ........................ 6 Introduction ........................................ ........................................ ........................................ ......... 7 Overview.

denatured lysate and then follow the hybrid protocol on page 20. Note that the hybrid protocol may not restore activity in all cases, and should be tested with your particular protein. 6. Remove 5 μL of the lysate for SDS-PAGE analysis. Store the remaining lysate on ice or freeze at –20°C. When ready to use, proceed to the protocol on page 16.

  Protocol, Ni nta