Tryptose Cycloserine Azide Agar Base - himedialabs.com

1 Please refer disclaimer Cycloserine Azide agar BaseM1279 Tryptose Cycloserine Azide agar base is recommended for enumeration of sulphite reducing anaerobes essentiallyClostridia .Composition**IngredientsGms / digest of soyabean ammonium pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclavingat 15 lbs pressure (121 C) for 15 minutes. Cool to 50 C and aseptically add ml rehydrated contents of 1 vial of (FD014) for 500 ml medium. Mix well and pour into sterile Petri : Sodium Azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enoughwater to flush off the And InterpretationTryptose Cycloserine Azide agar base was originally formulated by Hauschild and Hilsheimer (1). This medium was latermodified by decreasing the concentration of D- Cycloserine , sulphite and iron and by the addition of sodium Azide (2, 3).

2 Thismedium utilizes the selective inhibitory properties of D- Cycloserine and an indicator system involving sulphite and iron. Growthof non-mesophillic organisms are suppressed while C. perfringens and related species will reduces the sulphite and formblack colonies due to the production of ferrous sulphide (4). Tryptose , papaic digest of soyabean meal, meat extract and yeast extract provide essential nitrogenous compounds and vitaminsneeded for the growth of anaerobes. Glucose serves as carbon source. Disodium disulphite is reduced to hydrogen sulphidewhich combines with ferric ions of ferric salts to produce the insoluble black precipitate of ferrous sulphide. D- Cycloserine (FD014) and sodium Azide inhibit a number of organisms including Bacillus species, enteric bacilli, Proteus ,Pseudomonas and most of the cocci. Some anaerobes reduce sulphite to hydrogen sulphide (H2S) which is indicated byblackening of the colonies due to presence of ferric ammonium ControlAppearanceCream to brownish yellow homogeneous free flowing powderGellingFirm, comparable with agar and Clarity of prepared mediumYellow to amber coloured clear to slightly opalescent gel forms in Petri of w/v aqueous solution at 25 C.

3 PH : LaboratoriesTechnical ResponseM1279: Cultural response observed after an incubation at 35-37 C for 18-24 hours with added Supplement (FD014).OrganismInoculum(CFU)GrowthRecov eryColour ofcolonyClostridium perfringensATCC 1292450-100good>=50%blackClostridium sporogenesATCC 1143750-100good>=50%blackEscherichia coli ATCC25922>=10 inhibited0%Staphylococcus aureusATCC 25923>=10 inhibited0%Storage and Shelf LifeStore below 30 C in tightly closed container and the prepared medium at 2-8 before expiry date on the Hauschild A. H. W. and Hilsheimer R., 1974, Appl. Microbiol., 27, Eisgruber H., 1986, Vet Med. Diss. FU Eisgruber H. and Reuter G., 1991, Arch. Lebensmittelhyg, 42, Corry J. E. L., Curtis G. D. W. and Baird R. M., 1995, Culture Media for Food Microbiology, Vol. 34, ELSEVIER, : 1 / 2011 himedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India.

4 Customer care No.: 022-61471919 Email: :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related himedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. himedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.