Tca Protein Precipitation
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Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com• Precipitation has an advantage over dialysis or desalting methods in that it enables concentration of the protein sample as well as purification from undesirable substances. • One disadvantage of protein precipitation is that proteins might denature, making the pellet difficult to re-solubilize.
Fundamentals of Protein Chemistry - Duke University
genome.duke.eduTCA Precipitation: (if [protein] is >0.05 mg/mL) Chill protein in a microcentrifuge tube to 0°C . Add 1/9 volume of cold 100% w/v TCA. Vortex . Incubate at 0°C for 30-60 min. Spin down in microcentrifuge. Remove supernatant. Wash pellet 3x w/ 200 μL cold acetone (do not vortex)
Ni-NTA Purification System - Thermo Fisher Scientific
tools.thermofisher.comexpected yield of your protein and amount of resin to use for purification. ... or perform TCA precipitation prior to SDS-PAGE to prevent the precipitation of SDS. Harvesting Insect Cells For detailed protocols dealing with insect cell expression, consult the manual for your particular system. The following lysate protocols are for baculovirus-