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Search results with tag "Streptococcus pyogenes"
CRISPR 101
crispr.worksrestriction enzymes in concert with recombinant adeno-associated viruses (rAAVs). AAV is a non-pathogenic virus ... endonuclease protein (Cas9). The Cas9 protein functions as a pair of molecular ... the PAM for the most widely used Cas9 from Streptococcus pyogenes is 5′-NGG-3′, where N is any nucleotide. In its natural form, the gRNA ...
Protocols for Recombinant Cas9 protein …
www.toolgen.comtoolgen.com Page 1 Protocols for Recombinant Cas9 protein (Streptococcus pyogenes) 1. Reconstitution protocol for Cas9 protein (lyophilized).