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CRISPR 101
restriction enzymes in concert with recombinant adeno-associated viruses (rAAVs). AAV is a non-pathogenic virus ... endonuclease protein (Cas9). The Cas9 protein functions as a pair of molecular ... the PAM for the most widely used Cas9 from Streptococcus pyogenes is 5′-NGG-3′, where N is any nucleotide. In its natural form, the gRNA ...
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