0.1% Peptone Salt Solution - himedialabs.com

1 Please refer disclaimer Peptone salt Peptone salt Solution is used as diluent for different test **IngredientsGms / LitreBacteriological pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclavingat 15 lbs pressure (121 C) for 15 minutes validated And Peptone salt Solution is recommended as a diluent for dilution of sample by different test methods widely used forexamination of foodstuffs. Standard methods for the examination of foodstuffs require sample dilution to be carried outaccurately for enumerating the microorganisms. This medium is also recommended by ISO Committee (1) for use as an contains Peptone at low concentration which provides nutrients for survival of microorganisms and hence protecting theorganisms (2).

2 Sodium chloride at concentration maintains osmotic balance of medium thereby maintaining cellmorphology and integrity (3). The pH of this diluent medium is near neutral range optimum for viability of it can be successfully used as a diluent for carrying out dilutions of different is recommended to use 10 gm of test sample along with 90 ml of Peptone salt Solution for enumeration. The prepareddilution may be blended at 15,000 to 20,000 revolutions per minute. Further a ten fold dilution may be prepared using 1 ml ofit in 9ml of sterile diluent within 15 minutes and mixed well. This is considered as 101 -dilution. Sequential dilutions can beprepared using same diluent and counts obtained by spread plate or pour plate technique. Tests may be performed in duplicatesas described in technique and checked for equivalent yields of organisms between the diluent the tubes with test organisms.

3 At time of zero minutes and after 30 minutes and 2 hours, subculture an inoculum(approximately ) or a loop full onto Soyabean Casein Digest Agar (M290) using streak plate technique. If desired SCDAmay be also enriched with 5% v/v sheep blood depending on intended organisms to be isolated. Incubate plates at 35 2 Cfor 18-24 ControlAppearanceOff white to yellow homogeneous free flowing powderColour and Clarity of prepared mediumCream to pale yellow clear Solution in tubesReactionReaction of w/v aqueous Solution at 25 C. pH : ResponseCultural characteristics observed on Soyabean Casein Digest Agar (M290), after an incubation at 35-37 C for 18-48 hours ofcultures suspended in Peptone salt Solution for 30 ResponseHiMedia LaboratoriesTechnical DataOrganismInoculum(CFU)Recovery(after 30minutes)Cultural ResponseEscherichia coli ATCC2592250-100no change innumbersStaphylococcus aureusATCC 2592350-100no change innumbersStorage and Shelf LifeStore below 30 C in tightly closed container and the prepared medium at 2 8 C.

4 Use before expiry date on the Organization for Standardization (ISO),ISO/DIS Stokes , 1957, ,5 and Cassells , 1963, , 26 : 2 / 2015 HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time.

5 Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.