4.1.3. BUFFER SOLUTIONS - DrugFuture

1 EUROPEAN PHARMACOPOEIA BUFFER solutionsZirconium standard solution (1 g/L Zr). nitrate Requivalent to g ofZrO(NO3)2,2H2 Oinamixtureof2volumesofhydrochloricacid Rand 8 volumes ofwater Rand dilute to mL withthe same mixture of BUFFER SOLUTIONSB uffered acetone g ofsodium acetate Rand 42 g ofsodiumchloride Rinwater R, M hydrochloric acidand 150 mL ofacetone Rand dilute to 500 mL withwater solution pH g ofpotassium chloride Rinwater Rand mL M hydrochloric acid. Dilute to mL withwater BUFFER solution pH g ofdisodium hydrogen phosphate Rand gofpotassium dihydrogen phosphate Rinwater Rand diluteto mL with the same solvent. If necessary adjust thepH withphosphoric acid BUFFER solution pH g ofammonium sulfate Rinwater Rand diluteto mL with the same solvent (Solution A). Carefully andwith constant cooling stir 14 mL of sulfuric acid R into about400 mL ofwater R; allow to cool and dilute to mL withwater R(Solution B).

2 Mix equal volumes of SOLUTIONS A and the pH if solution pH mL ofphosphoric acid Rwith mL of a 40 g/Lsolution ofsodium hydroxide Rand dilute to mL withwater solution pH 100 g ofpotassium dihydrogen phosphate Rin800 mL ofwater R; acid Rand dilute to mL withwater solution pH g ofdilute phosphoric acid Radd 250 mL ofwater the pH withdilute sodium hydroxide solution Randdilute to mL withwater BUFFER solution pH g ofsodium dihydrogen phosphate Rin 900 mL ofwater R, acid Rand dilute to1000 mL with the same solution pH g ofcitric acid Rin 200 mL of1 Msodiumhydroxideand dilute to 1000 mL withwater R. Dilute mLof this solution to mL M hydrochloric M Citrate BUFFER solution pH g ofcitric acid Rin 80 mL ofwater with1 M sodium hydroxideand dilute to mL withwater M Phosphate BUFFER solution pH g ofanhydrous sodium dihydrogen phosphate Rinwater R,adjustthepHwithdilute phosphoric acid R1anddilute to 1000 mL withwater BUFFER solution pH mL ofphosphoric acid Rwith 100 mL ofwater to 900 mL with the same solvent.

3 Adjust to pH withstrong sodium hydroxide solution Rand dilute to 1000 mLwithwater BUFFER solution pH g ofpotassium dihydrogen phosphate Rin900 mL ofwater acid Rand dilute to mL withwater BUFFER solution pH 900 mL of a 4 g/L solution ofsodium dihydrogenphosphate R, BUFFER solution pH a g/L solution ofdisodium hydrogen phosphate Rto pH withdilute phosphoric acid to mL withwater solution pH g ofammonium acetate Rin 25 mL ofwater Rand add mL ofhydrochloric acid if necessary withdilute hydrochloric acid Rordiluteammonia R1. Dilute to mL withwater BUFFER solution pH g ofpotassium dihydrogen phosphate RinwaterRand dilute to mL with the same solvent. Adjustthe pH withphosphoric acid solution pH mL M potassium hydrogen phthalate mL M hydrochloric acid. Dilute to mL withwater solution pH mL ofacetic acid Radd 60 mL ofethanol (96 percent) Rand 20 mL ofwater R. Adjust to pH by the additionofammonia R.

4 Dilute to mL withwater copper sulfate solution pH g ofcopper sulfate Rand g ofammoniumacetate Rindilute acetic acid Rand dilute to mL acetate BUFFER solution pH ( M). 822 mg ofsodium acetate Rin 100 mL ofwater R(solution A). Dilute mL ofglacial acetic acid Rin 250 mLofwater R(solution B). Titrate 100 mL of solution B usingabout 20 mL of solution BUFFER solution pH 136 g ofsodium acetate Rand 77 g ofammoniumacetate Rinwater Rand dilute to mL with the samesolvent; add mL ofglacial acetic acid Rand BUFFER solution pH g ofpotassium hydrogen phthalate Rin 50 mLofwater R, M sodium hydroxideand dilute mL withwater BUFFER solution pH g ofammonium acetate Rinwater acetic acid Rand dilute to mL withwater M Phosphate BUFFER solution pH g ofpotassium dihydrogen phosphate mL ofwater ( )of the solution is acetate BUFFER solution pH 63 g ofanhydrous sodium acetate Rinwater R,add90 mLacetic acid Rand adjust to pH , and dilute to 1000 mLwithwater BUFFER solution pH g ofsodium acetate Rin 50 mL ofwater R, g ofglacial acetic acid Rand dilute to mL withwater Notices (1)

5 Apply to all monographs and other BUFFER solutionsEUROPEAN PHARMACOPOEIA BUFFER solution pH g ofsuccinic acid Rin a mixture of 600 mL ofwater Rand 82 mL of1 M sodium hydroxideand dilute mL withwater BUFFER solution pH g ofsodium acetate Rin 500 mL ofwater 250 mL of this solution with 250 mL ofdilute aceticacid R. Shake twice with a freshly prepared, filtered, g/Lsolution ofdithizone Rinchloroform Runtil the extract is colourless. Filter theaqueous layer to remove traces of carbon BUFFER solution pH g ofsodium acetate Rin 500 mL ofwater mL of this solution with 250 mL ofdilute acetic acid BUFFER solution pH acetic acid Radd100 mL M potassium hydroxideand about 250 mL ofwater R. Mix. Adjust the pH to with a 6 g/L solution ofacetic acid Ror M potassium hydroxideand dilute mL withwater BUFFER solution pH a solution containing g/L ofcitric acid g/L ofsodium hydroxide acid BUFFER solution pH g ofpotassium dihydrogen phosphate Rin800 mL ofwater dilute to 1000 mL withwater solution pH g ofpotassium hydrogen phthalate Rin M sodium hydroxide.

6 Dilute to mL withwater M Phosphate BUFFER solution pH appropriate volumes of a g/L solution ofdisodiumhydrogen phosphate Rwith a g/L solution ofsodiumdihydrogen phosphate monohydrate Rto obtain pH ( ).Acetate-edetate BUFFER solution pH 250 g ofammonium acetate Rand 15 gsodiumedetate Rin 400 mL ofwater Rand add 125 mL ofglacialacetic acid solution pH g ofsodium acetate Rin 50 mL ofwater R,heating to 35 C if necessary. After cooling, slowly add 10 mLofanhydrous acetic acid R. Shake and dilute to mL withwater BUFFER solution pH g ofpotassium dihydrogen phosphate Rinwater Rand dilute to mL with the same solvent(solution A). Dissolve g ofdisodium hydrogenphosphate Rinwater Rand dilute to mL with the samesolvent (solution B). Mix mL of solution A and mL ofsolution BUFFER solution pH mL of a g/L solution ofanhydrous disodiumhydrogen phosphate Rand mL of a 21 g/L solution ofcitric acid BUFFER solution pH g ofpotassium dihydrogen phosphate Rinwater (solution A).

7 Dissolve g ofdipotassium hydrogenphosphate Rinwater Rand dilute to mL with the samesolvent (solution B). Mix mL of solution A and mL ofsolution B. If necessary, adjust to pH using solution A orsolution BUFFER solution pH g ofdisodium hydrogen phosphate dihydrate Rand g ofpotassium dihydrogen phosphate Rinwater Rand dilute to mL with the same BUFFER solution pH 100 g ofammonium acetate Rin 300 mL ofwater R,add mL ofglacial acetic acid R,adjustthepHifnecessaryusingammonia Roracetic acid Rand dilute to mL withwater phosphate BUFFER solution pH 68 mL ofphosphoric acid Rto 500 mL withwater 25 mL of this solution add 450 mL ofwater Rand6 mLofdiethylamine R, adjust to pH 6 , if necessary, usingdiethylamine Rorphosphoric acid Rand dilute to mLwithwater BUFFER solution pH mL of a g/L solution ofdisodium hydrogenphosphate Rand mL of a 21 g/L solution ofcitric acid BUFFER solution pH g ofsodium dihydrogen phosphate Rinwater Rand dilute to mL withwater sodium hydroxide solution BUFFER solution pH mL M potassium dihydrogen phosphate mL M sodium hydroxideand dilute to mLwithwater BUFFER solution pH g ofdisodium hydrogen phosphate R, dihydrogen phosphate Rand g ofsodiumchloride Rin 950 mL ofwater R.

8 Adjust the pH of the solutionto with1 Msodiumhydroxideor1 Mhydrochloricacid,ifnecessary. Dilute to mL withwater M Phthalate BUFFER solution pH 100 g ofpotassium hydrogen phthalate Rinwater Rand dilute to mL with the same solvent. Adjust the pH ifnecessary, usingstrong sodium hydroxide solution solution pH g ofdisodium hydrogen phosphate Rand 46 g ofpotassium dihydrogen phosphate Rinwater M sodium edetateand 20 mg ofmercuric chloride Randdilute to mL withwater BUFFER solution pH g ofimidazole R, sulfate Rand g ofcalcium sulfate Rin 752 mL M hydrochloricacid. Adjust the pH if necessary and dilute to mL withwater phosphate BUFFER solution pH g ofsodium dihydrogen phosphatemonohydrate Rin 900 mL ofdistilled water a 400 g/L solution ofsodium hydroxide mL withdistilled water BUFFER solution pH g ofsodium dihydrogen phosphate Rand gofsodium chloride Rin 500 mL ofwater BUFFER solution pH solution pH mL M potassium dihydrogen phosphate mL M sodium hydroxide.

9 Dilute to mL withwater the information section on general monographs (cover pages)EUROPEAN PHARMACOPOEIA BUFFER solutionsPhosphate buffered saline pH g ofpotassium dihydrogen phosphate R, hydrogen phosphate Rand g ofsodiumchloride Rin 900 mL ofwater R, adjust the pH if necessary anddilute to mL with the same BUFFER solution pH hydrogenphosphate Rwith mL of a 21 g/L solution ofcitric acid BUFFER solution pH mL of a g/L solution ofpotassium dihydrogenphosphate Radd mL of a g/L solution ofdisodiumhydrogen phosphate R. Adjust the pH if :at2 Cto8 M tris-hydrochloride BUFFER solution pH g oftris(hydroxymethyl)aminomethane Rin400 mL ofwater acid Randdilute to mL withwater solution pH 1000 mL of a solution containing 18 g/L ofdisodiumhydrogen phosphate Rand 23 g/L ofsodium chloride Raddsufficient (about 280 mL) of a solution containing g/Lofsodium dihydrogen phosphate Rand 23 g/L ofsodiumchloride Rto adjust the pH.

10 Dissolve in the solution sufficientsodium azide Rto give a g/L BUFFER solution pH g ofsodium chloride R, (hydroxymethyl)aminomethane Rand g ofmaleicanhydride Rin 900 mL ofwater g/L solution ofsodium hydroxide R. Dilute to mLwithwater :at2 Cto8 C; M Phosphate BUFFER solution pH 1 volume M phosphate BUFFER solution pH Rwith volumes ofwater M Phosphate BUFFER solution pH g ofdipotassium hydrogen phosphate Rin900 mL ofwater for chromatography acid Rand dilute to 1000 mLwithwater for chromatography M Phosphate BUFFER solution pH 34 mL ofwater Rand 100 mL M phosphate buffersolution pH M Phosphate BUFFER solution pH g ofanhydrous disodium hydrogen phosphate Rand g ofsodium dihydrogen phosphate monohydrate Rin950 mL ofwater Rand adjust the pH withphosphoric acid R;dilute to mL withwater M Phosphate BUFFER solution pH g ofpotassium dihydrogen phosphate Rinwater (solution A). Dissolve g ofdisodium hydrogen phosphate Rinwater (solutionB).