Transcription of 使用 Agilent 6550 Q-TOF 质谱仪 进行完整蛋白质分析
1 Agilent 6550 Q-TOF LC/MS (PTM) PTM Agilent 6550 Q-TOF Ravindra Gudihal Suresh Babu CV Bangalore IndiaNing Tang Santa Clara, CA USAM adhavi Uma Maheswari GangaGen Biotechnologies Pvt. Ltd Bangalore, India2 (FA) 3% (ACN) (mAb) 100 g/mL 1 L (100 ng) ( p128 ) GangaGen Biotechnologies Pvt. Ltd (TFA) 3% (ACN) Agilent 1290 Infinity Agilent 6550 iFunnel Q-TOF 1 48 mAb Agilent MassHunter BioConfirm 2 1.
2 MAb LC/MS Agilent 1290 Agilent Poroshell 300SB C8 75 mm, 5 m Agilent ZORBAX RRHD 300 Diphenyl, 100 mm, m 858750-944 80 C 60 C 5 C TFA 90% 0-1 min B 3% 1-5 min B 3% 90% 5-6 min B 90% 6-7 min B 3% 7 mL/minLC/MS ESI (Profile) 290 C 14 L/min 400 C 12 L/min 20 psi 5000 V 2000 V LC/MS Agilent MassHunter Agilent MassHunter BioConfirm 3 mAb 3 4 p128 26490 Da Agilent 1290 Infinity Agilent 6550 iFunnel Q-TOF Agilent 1290 Infinity Agilent 6550 iFunnel Q-TOF Agilent MassHunter BioConfirm 2.
3 MAb mAb 3. p128 LC/MS p128 4. p128 2013 2013 3 29 5991-2116 CHCN 800-820-3278 400-820-3278
