Transcription of Bacillus cereus
1 Bacillus cereus 1) 2)1) 2) ! 16 8#23 17 4#25 2002 '( ( Bacillus cereus -. 0 2 3 6 78 2 3 B. cereus 0 " # < B. cereus > @ ) 78 +, - 2C D0 01 3 EFC G 0J KL 0 M CO 5P QR S7 T 9 G V W B. cereus XY Z[ ;L0\<= W ^B. cereus ? @A 0 B ` 60 75 cd eE f V D02A C k F JeE = l 0 KL 0mK G W n B. cereus o 0N P BacillusR qrsS=TV n VU t 6 103 104 CFU/ml u Wv0 W Y G V B. cereus 0 104 CFU/ml VU 0 [ \ yz{ v ` W Key words: Bacillus cereus ,eEV ` Bacillus cereus eE| = qrsS=TV 9 }a~XY @b XYc 7 W Rh V W B. cereus k qr = V J G V @A V W 1), 2) BacillusR j kl u r Q p3 4 W n f C G V BacillusR eE k F @> s = l 0 k v P G V w 3) 5) 2002 8, 9# 23#t 6x 2 3 6 78 2 3 B.]]
2 cereus 0 XY ICT (Infection Control Team) 0 u r E 0 y 78 u r ?N B. cere-us h }2 3 6 { G V KL 0 ^ Rh } 3 V | J ~2 v0h XYV G 06) [ \ yz{ W Centers for DiseaseControl and Prevention (CDC) 7) [ E 0c 7! B. cereus n P V 8 ( 448 8505) 5 15 TEL: 0566 25 2946 FAX: 0566 25 8216E-mail: [ c 7! Vol. 15 No. 2 Q I. 1. 2002 1 2003 12 !" $ ' ( ' * *+ ,- * 0!" 1 0 1 2 3 *4 56!" 5 !1 " ' * 7 " 56 5 $8!" 9 % 5 :' ;<* ! = + ? ,-" 5 *1 * + = B FG " 4 ! H . JK ! H . M / 1B * * 2O3J 1 3 * ! H . <* :6 ( 3 ) Q S8 * 2. 1) 5 9: 2 * 1 ! H . 9 " U= V?]]]
3 * :M V M 20@JA Z *% B \ C * " 1_ !1 *: `a bc d J+ `a e bc "f * d g `a Fh ij* * M G9 Hl *j 9 " = m *2 Fh 200 g n 1 200 g "f l J5G Ko * L 10qJA r sJ5G N * d t O2 uv % +Q" RsG9 9 T d 0 G9 5UV * 2) FX 5UV %" 30ml z }Y Z G \ !" ] Z G \ d g K _ h 37 2 U *0 BQ 2 * d 0 ` v 6 6 5 ab(CFU/ml) ij* 5 ab(CFU/ml) X 100030X:z }Y ZA $ * v 6 TGC F de Z G \ 9ml 1ml 5UV Ko * !56 50 CHB, 20E 2u * 3)1 h i1 1 0 2 j 0 1 20@ O2 uv +U "f lm *: 3 Z no !4 BQ !15 22 pqG !37 d g 24 * * . " 5UV 20 ml bc * d 0 d g bc 65 359 ou= m * ou= "< : J <*F vwx5 ` <* h *8) d ou= 0 bc % " 30ml F *FX * ` * v 6 6 ` FX ` * h ab(CFU/ml) ij* II.
4 1. ! # # ( * - 0 B. cereus2002 1 2003 12 ' - * B. cereus w = {6607{ = 2002 8, 9 d 10 } BK - ~ <* $ m;- + = - % $ B. cereusvw- 6ij * 2002 10 K " ; $ 2003 2 " b; ; d `a 2003 B. cereus ' 2003 9 23 K * 1 2003 2 m * j* = + ? ,- "f * ab "f N *80 u 109 d " Bacillus cereus " Bacillus cereus " 2 { Vol. 15 No. 2 100 , " '() +,- ./ 01 2 +,3 45267 :; => (, ? B : ICUF F=> IJKL ) :: 0,IJ MNO5PQ S TU 10F IJK V XY Z ] ^ 0; 2) IJ B : ICU F IJK 100 ,2) MNO5 d IJ B : 2. 1) ef 5g:5h2003i5 ; ki4 lm5g n pq s2 i u wx: 0 103 104 CFU/ml 5g2z |Y, 5s1 ICT Y- ; B. cereus F w Bacillus F :B.}}
5 cereus ,: Bacillus ( 5 45 F 350 2002i d s22003i5 ; 2004i4 IJ B 5g |Y, 5g 1, 5g B 5g B ICU 5g , Q Vol. 15 No. 2 104 CFU/ml ! # '( )* 1+( - .-(2 3 0 10 1 3 45 2003697 .-65 80 -25 29 ( < = 103 CFU/ml 1+ )! 104 CFU/ml ?45 1+ )! 2004617@A BC D? E 0< F? G HI( L M D M5 Q5R S5( 5 5TU ? )XY 6Z [ 4 - 20 26 ,.- 55 85 ( ? ]^ ` =a B. cereus , B. subtilis, Paeniba-cillus polymyxa cd Type strain DNA/DNAhybrdization ef E = ]^ B. cereus k D? no 3a = 71p 16Z BCqZ* Dr L 7ef ` 2) ICU, s(t vw Dr ]^=ICU( ` vw 2003657 vwx 100 ,3<Z yz{= |} ~ `@ Bacillus ]^Q ? 3E ' + 5 3 = / + A) E !( =@ efQ( D? B)E 1+ !( 5 P. polymyxa cd C) 2+ ! = f D) 3+ ! = =Q ? D)Dr = 3a ef Bacillus cereus vw ) 54 Bacillus cereus vw ) 54+ Q Vol.)
6 15 No. 2 ! "# %75 '( ) + %,100 . , " /0 Q 4 102 103 CFU/ml "8 :2 7, 8, 10= > @ B E @ 1FG I J#K G L M KN OP QR T U I J V B WX8 Z .\ #_ @ G `a c Q 4 %d21 26 ,N%d50 76e "8 3) #_ @ c G \ f gh \ i E @ #_ @ kI Bacillusl J U n 103 CFU/mlpq U I2F d10 O+ 3Fs tq Z .\ x { 105 CFU/ml|W O M % :3 G % d16 21 ,N%d45 60e "8 4) @L % @ J ) + I @ B 100 () + ! 60 75 :5 I % @ B f % B i 1Xs B i 2Xs:4 % @ J ) + I @ B ! J I % | # " A, B ! 95 W U Id60 C, D ! 100 40 ' U Id70 E, F, G, H, I ! 75 W U I75 ( ' J, K, L, M ! 100 ( ' F Vol. 15 No. 2 103 104 CFU/ml 4 Q 2 3% ( ) ( + , + - ! 75 #$ 3 / & 0 ' 1/5 5 /(& 2 5 3.}
7 4) 5 6+ 1,-7. 89 + ;< = 2 2 1,-7 6+ > 45 0 / 6 4 7 15 22 8@ 37 9 A 0 B 104 CFU/ml, 104 CFU/ml, 104 CFU/ml 2 ;<45 =D G 6+ K+ @ 6+ 5 III. LM NO R T CV DE , B. cereus G WH I X LM NO Y>45ZY [ R T K ^ _V b LMB. cereus W X Y [G 60 75 ]Y . 6+( e f h + [ Y>Z O ` 6+ b b [ B. cereus Y7'T kcld e mT no pg 45 ^ V +&V 2 9) ,Q t jk ` 80 ,10 vQ + lw x |m } ]Y ~ o + p/ [ 103 104 CFU/ml r 4) 0 [ 6+ 4) b=6 t -7 / 7Y (& 2 b W W 6+A I A E -7 x 2 Bacillus subtilis 6+ j h < 85 A 2 [ |} |m } 6+ 5 ' h pH 9 ~ - 500 ppm |m (& + 8) CDC t W + 160 71 25 A ~ ~b + 7) lw + Y>45 B. cereus _ K 3 l 104 CFU/ml 2 lw & + + (& E -7 A h 0 b vQ + W } t WH ^ - 2 [G, 2 60 75 6+( e f h A Ba-cillus subtilis ,100 - 6+ 1/10 90 (& / + 8) ;< 100 30 6+ 100 60 ]Y _ 2 _ 100 120 _ A g lw ICU, X + X + 100 W ICU + 100 3 / ]Y [ L 100 2~ 85 60 2 2 _ NO R T V lw t w b 89 2 A w' 7 NO R T l + 45 2002p10 131.]]]]]
8 13 _ A _ w t ^ 2 G ( (& b L = Q O R T + 7Y7 ICT w 7Y + (& 2 Bacillus 6+ v Bacillus cereus NO R T 7Y + Bacillus cereus NO R T 7Y + ,Q Vol. 15 No. 2 Q #$%&'( )*#$'( ,( -. 2 45 5 Paenibacillus polymyxa 849 ;<=> ?@ A CDE I D 2 45 K LM N4 O 1) Souvenir, D., D. E. Anderson, Jr., S. Palpant, etal. 1998. Blood cultures positive for coagulase-negative staphylococci: antisepsis, pseudobac-teremia, and therapy of patients. J. Clin. Micro-biol. 36: 1923 ) Qian, Q. F., Y. W. Tang, C. P. Kolbert, et Direct identification of bacteria frompositive blood cultures by amplification andsequencing of the 16S rRNA gene: evaluationof BACTEC 9240 instrument true-positive andfalse-positive results. J. Clin. Microbiol. 39:3578 ) Liu, P. Y., S. C. Ke, S. L. Chen. 1997. Use ofpulse-field gel electrophoresis to investigate apseudo-outbreak ofBacillus cereusin a pedi-atric unit.))
9 J. Clin. Microbiol. 35: 1533 ) Hsuh, P., L. Teng, P. Yang, H. Pan, S. Ho, K. Nosocomial pseudoepidemic caused byBacillus cereustraced to contaminated ethylalcohol from a liquor factory. J. Clin. : 2280 ) Barrie, D., P. N. Ho#man, J. A. Wilson, J. M. Kra-mer. 1994. Contamination of hospital linen byBacillus Infect. 113: 297 )P;QR 2003. T VW #$YZ[%45 ]^_%` p. 14 33,a D ef 5#$,( h3i j l mn p rs P;QR t) w yD { | 7) CDC: Guidelines for Environmental InfectionControl in Health-Care Facilities Recommenda-tions of CDC and the Healthcare Infection Con-trol Practices Advisory Committee (HICPAC)2003. June 6, Vol. 52/No. RR-10. )~ 1994. O 45: 63 ) Oosthuizen, M. C., B. Steyn, J. Theron, et Proteomic analysis reveals di#erentialprotein expression byBacillus cereusduringbiofilm formation. Appl. Environ. : 2770 ofBacillus cereusPseudobacteremia Linked toContaminated WashclothsYoshio Izawa,1)Makoto Ito2)1)Divisions of Clinical Microbiology,2)Infection Control and Epidemiology, Department of Pathology andLaboratory Medicine, Toyota Medical Corporation, Kariya GeneralHospital, 5 15 Sumiyoshi, Kariya, Aichi 448 8505, JapanDuring the summer and autumn seasons of 2002 in an acute-care tertiary hospital, an outbreak ofpseudobacteremia caused byBacillus cereusoccurred in chronically ill patients with an indwellingperipheral venous catheter.}
10 The source ofBacilluscontamination was examined by analyzing extensivesurveillance cultures of the patient s skin, fabrics, medical equipment, and environmental surfaces. Anunexpectedly high level ofB. cereuscontamination was identified in washcloths that were routinely usedin nursing practices, such as backrubs, towel baths, and perineal care. The laundry of washcloths wasoutsourced. Clean, dry washcloths that were supplied following washing and sodium hydrochloridedisinfection, were then soaked in water and heated in a steam bath at 65 70 in the hospital ward beforebeing used for care. Under these conditions, we consistantly found 103 104 CFU/ml ofBacillusspecies inthe freshly prepared washcloths. Up to 106 CFU/ml of contaminating microorganisms could be recovered p p ]^_%` Vol. 15 No. 2 washcloths stored at ambient temperature for more than 72 hours. It was postulated that theBacilluscereuspresent in these washcloths might be transferred to the patient s skin during sanitary care, whichwould then cause contamination of blood culture specimens following needle sticks.
