CHAPTER 1 INTRODUCTON Histopathology - rajswasthya.nic.in

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CHAPTER 1 INTRODUCTON Histopathology - …

1 CHAPTER 1 INTRODUCTON Histopathology - Definition it is a branch of pathology which deals with the study of disease in a tissue section. The tissue undergoes a series of steps before it reaches the examiners desk

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bacterial cultures 3 Staining Techniques (Simple, Gram staining, and spore staining) ... 12 Identification of Bacterial morphology by phase contrast Microscopy/Live and dead ... 6 Detergents Disrupt cell membrane Denature protein & disrupt cell 7 Phenols membrane

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1. Bacterial cell (morphology, staining reactions, classification of bacteria) The protoplast is bounded peripherally has a very thin, elastic and semi-permeable cytoplasmic membrane (a conventional phospholipid bilayer). Outside, and closely covering this, lies the rigid, supporting cell wall, which is porous and relatively permeable.

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staining; and 3) will retain the original cell morphology after fixation and staining. After you have stained your bacterial smears, you will examine them with the oil immersion lens, noting the morphological and staining characteristics of each species. The usual bacteria that you see range in size from 0.5 to nearly 10 microns (a few

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Prior to staining with dyes, cells must be "fixed." Fixation is a process of killing, immobilizing and preserving the bacterial cell. For bacteria, heat fixation is most common; however, chemicals such as formaldehyde, glutaraldehyde, acids and alcohols can be used. Dyes used to stain bacterial cells are organic compounds which have

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Conklin method where malachite green is used for staining and heat is used to penetrate stain. The rest of the cell is then decolorized and counterstained a light red with carbolfuchsin. Fig. 12 C. botulinutm, Wirtz-Conklin stain. C. botulinum: these gram-positive bacilli have subterminal spores (no terminal spores).

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MORPHOLOGY AND Notes CLASSIFICATION OF BACTERIA

MICROBIOLOGY MODULE Morphology and Classification of Bacteria Microbiology 2 Notes zMost bacteria possess peptidoglycan, a unique polymer that makes its synthesis a good target for antibiotics zProtein synthesis takes place in the cytosol with structurally different ribosome’s Fig. 1.1: Prokaryote Cell Fig. 1.2: Eukaryote Cell Difference between Prokaryotic and …

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