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Flow Cytometry (FACS) Protocols
I. Preparation of human peripheral blood mononuclear cells (PBMC) Leukocytes are the most commonly analyzed cells in flow cytometry. Leukocytes can be obtained from whole blood and a variety of tissues, such as spleen, lymph node, bone marrow and thymus. Red blood cells (RBC) present in whole blood or cell preparations are a contaminant
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