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PBMC Isolation from Whole Blood by Density Gradient …
Isolation of mononuclear cells Yellowish layer of dilute plasma and platelets Fluffy white layer of PBMCs at the interphase Ficoll or cell-separation medium RBC and granulocyte layer FIGURE 2. Separation of whole blood using Ficoll. 45 40 35 30 25 20 15 10 mL 45 40 35 30 25 20 15 10 mL Diluted blood sample Ficoll separation medium 45 40 35 30 ...
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