免疫细胞分型指南 - Thermo Fisher Scientific

1 Thermo Fisher Scientific Invitrogen Attune NxT eBioscience Super Bright PrimeFlow RNA RNA ContentsB cell markers 4CD4 T cell markers 6CD8 T cell markers 8 Dendritic cell markers 10 Granulocyte markers 12 Macrophage/monocyte 14 Megakaryocyte/platelet markers 16NK/ILC markers 18NK T/ T cell markers 20 Endothelial cell markers 22 Stem cell markers 24 Other cell type markers 26 Human CD antigens 28 Human non-CD antigens 52 Mouse CD antigens 57 Mouse non-CD antigens 76 Abbreviations Act Activated ASV Alternative splice variants ADCC Antibody-dependent cellular cytotoxicity AML Acute myeloid leukemia APC Antigen presenting cells BM Bone marrow CCRSF Complement component receptor superfamily CD Cluster of differentiation CHO Carbohydrate moiety CNS Central nervous system CRSF Cytokine receptor superfamily CTL

2 Cytotoxic T lymphocytes DC Dendritic cells ECM Extracellular matrix Endoth Endothelial cells Epith Epithelial cells ESC Embryonic stem cells FDC Follicular dendritic cells GPI Glycophosphatidylinositol Gran Granulocytes HEV High endothelial venule HUVEC Human umbilical vein endothelial cells HSC Hematopoietic stem cells IgSF Immunoglobulin superfamily ILC Innate lymphoid cell Intra/Txn Intracellular/transcription factors kDa Kilodalton KO Knockout LAK Lymphokine activated killer Leuk Leukocytes LPS Lipopolysaccharide LRRF Leucine rich repeat family Lymph Lymphocytes Mac Macrophages MHC Major histocompatibility complex Mono Monocytes NK Natural killer pbmc peripheral blood mononuclear pDC Plasmacytoid dendritic cell PNS peripheral nervous system RBC Red blood cells RTK Receptor tyrosine kinase Tfh Folicular T helper cells Th T helper cells TF Transcription factor TM Transmembrane TM12SF 12-transmembrane spanning protein superfamily TM4SF 4-transmembrane spanning protein superfamily TM7SF

3 7-transmembrane spanning protein superfamily TNFRSF TNF receptor superfamily TNFSF TNF superfamily Format abbreviations FG purified Functional grade purified (sterile and azide-free) FG biotin Functional grade biotin (sterile and azide-free) FITC Fluorescein isothiocyanate PE Phycoerythrin PE-eFluor 610 Phycoerythrin-eFluor 610 Tandem PE-Cyanine5 Phycoerythrin-Cyanine5 Tandem Peridinin Chlorophyll Tandem PerCP-eFluor 710 Peridinin Chlorophyll Protein-eFluor 710 Tandem PE-Cyanine7 Phycoerythrin-Cyanine7 Tandem APC Allophycocyanin APC-eFluor 780 Allophycocyanin-eFluor 780B cell markersHumanFollicularSurfaceCD20CD21CD2 2CD27CD23h CD24CD40hCD45R (B220)CD69hCD80hCD81CD86hCD137 (4-1BB)hC D 275 ( B7- H 2)CD279 (PD-1)CD360 (IL-2 receptor)

4 HLA-DRIgDhigh IgMlow/intIntracellular/transcription factorBcl-6 EBF1 FoxO1 IkarosPax5 Marginal zoneSurfaceCD1dCD9CD21 CD45R (B220)CD81CD103CD180 (RP105)CD268 (BAFF receptor) CD307d (FcRL4)IgMIntracellular/transcription factorEBF1 Notch2 Pax5 MemorySurfaceCD27 IgGTLR1, 2, 6, 7, 10 Intracellular/transcription factorCBF1 Pax5 Spi-BGerminal centerSecretedIgAIgEIgGSurfaceCD45R (B220)CD81 Intracellular/transcription factorAIDBach2 Bcl-6 IRF8NF-kappaBPax5 Plasma cellSecretedIgAIgEIgGSurfaceCD9CD19low/n egCD20low/negCD27CD31CD38CD81CD138 CD184 (CXCR4)CD252 (OX40 ligand)CD269 (BCMA)Intracellular/transcription factorBLIMP1 IRF4X B P-1 Pan markersCD19 CD20 CD22CD70hCD79 / Ig / 4h = Upregulated L = Downregulated = Key marker = SubsetFollicularSurfaceCD20CD21/CD35low/ negCD22CD23 CD24 (HSA)lowCD25hCD36hCD40hCD69hCD80hCD81CD8 6hCD180 (RP105)CD252 (OX40 ligand)hIgDhigh IgMlow/intIntracellular/transcription factorBcl-6 EBF1 FoxO1 IkarosPax5 Marginal zoneSurfaceCD1dCD5low CD9CD21/CD35high CD23low/negCD24 (HSA)lowCD36CD81CD180 (RP105)CD363 (S1PR1)

5 FcRL3 IgDlow/negIgMhigh Intracellular/transcription factorEBF1 Notch2 Germinal centerSurfaceCD19CD81CD95GL7 IgGIntracellular/transcription factorAIDBach2 Bcl-6 IRF8NF- BPax5 Plasma cellSurfaceCD9CD16/32CD19lowCD81CD138 IgGIntracellular/transcription factorBLIMP1 IRF4X B P-1 Pan markersCD20CD21/CD35low/negCD22CD23CD24 (HSA)lowCD25hCD36hCD40hCD69hCD80hCD81CD8 6hCD180 (RP105)CD252 (OX40 ligand)hIgDhighIgMlow/intMouseB cell markersMemorySurfaceCD38 CD45R (B220)lowCD267 ( TACI)CD269 (BCMA)IgGIntracellular/transcription factorCBF1 Spi-B5 TfhSecretedI L- 2 1 SurfaceCD183 (CXCR3) CD185 (CXCR5) CD278 (ICOS) CD279 (PD-1) Intracellular/transcription factorBATFBcl-6c-MafIRF4 STAT3 TregSecretedI L-1 0 TGF SurfaceCD25 CD39CD73CD101CD121aCD121b CD137 (4-1BB)CD152 (CTLA-4)CD357 (GITR/AITR)GARPhLAPhTIGIT Intracellular/transcription factorc-RelEosFoxp3 HeliosSTAT5Th1 SecretedIFN I L- 2 TNF TNF (LT )SurfaceCD94C D119 ( I F N R1)CD183 (CXCR3) CD186 (CXCR6)CD191 (CCR1)CD195 (CCR5)CD212 (IL-12R 1)CD218a (IL-18R )CD254 (RANKL)CD366 (TIM3)

6 Intracellular/transcription factorSTAT1 STAT4T-bet Th17 SecretedGM-CSFI L-17AI L-17A FI L-17 FI L- 2 1I L- 2 2 SurfaceCD121aCD161CD194 (CCR4)CD196 (CCR6) C D 3 6 0 ( I L- 2 1R )CD212 (IL-12 1)I L- 2 3 RIntracellular/transcription factorAHRBATFc-MafI Bч IRF4 ROR ROR t STAT3Th2 SecretedAmphiregulin I L- 4I L- 5 I L-13 SurfaceCD184 (CXCR4)CD194 (CCR4)CD198 (CCR8)CD294 (CRTH2) CD365 (TIM1)I L- 2 5 R ( I L-17 R B )IL-33R (ST2)Intracellular/transcription factorBATF G ATA-3 IRF4 STAT6 Pan markersCD2CD3CD4CD5CD7CD25hCD27CD28CD45R A (na ve)CD45RO (memory)CD62L (na ve: high, effector: low, memory.)

7 High)CD69hCD127 (IL7R )CD134 (OX40)hCD137 (4-1BB)hCD152 (CTLA-4)hCD154 (CD40L)hCD272 (BTLA)hCD279 (PD-1)hCD4 T cell markersHuman6 TfhSecretedI L- 2 1 SurfaceCD185 (CXCR5) CD278 (ICOS) CD279 (PD-1) Intra/TxnBATFBcl-6c-MafIRF4 STAT3 TregSecretedI L-1 0 TGF SurfaceCD25 CD137 (4-1BB)CD39CD73CD101CD121aCD121bCD152 (CTLA-4)CD304 (Neuropilin-1) CD357 (GITR)FR4 GARPhLAPhTIGITI ntracellular/transcription factorc-RelEosFoxp3 HeliosSTAT5Th1 SecretedIFN I L- 2 TNF TNF (LT )SurfaceCD94C D119 ( I F N R1)CD183 (CXCR3) CD186 (CXCR6)CD191 (CCR1)CD195 (CCR5)CD212 (IL-12R 1)CD218a (IL-18R )CD254 (RANKL)CD366 (TIM3)Intracellular/transcription factorSTAT1 STAT4T-bet Th17 SecretedGM-CSFI L-17AI L-17A FI L-17 FI L- 2 1I L- 2 2 SurfaceCD194 (CCR4)CD196 (CCR6) CD121a CD212 (IL-12 1)C D 3 6 0 ( I L- 2 1R )I L- 2 3 RIntracellular/transcription factorAHRBATFc-MafI Bч IRF4 ROR ROR t STAT3Th2 SecretedAmphiregulin I L- 4I L- 5I L-1 0 I L-13 SurfaceCD184 (CXCR4)CD194 (CCR4)CD198 (CCR8)CD294 (CRTH2)CD365 (TIM1)I L- 2 5 R ( I L-17 R B )IL-33R (ST2)

8 Intracellular/transcription factorBATF G ATA-3 IRF4 STAT6 Pan markersCD3CD4CD5CD7CD25h CD27CD28CD44hCD62L (na ve: high, effector: low, memory: high)CD69hCD127 (IL7R )CD134 (OX40)hCD137 (4-1BB)hCD152 (CTLA-4)h CD154 (CD40L)hCD272 (BTLA)hCD278 (ICOS)hCD279 (PD-1)hMouseCD4 T cell markersh = Upregulated L = Downregulated = Key marker = Subset7CD8 T cell markersHumanCentral memorySecretedIFN int I L- 2intTNF intSurfaceCCR7lowCD27CD28CD45 ROCD62L CD62 LlowCD127 (IL7R )highCD197 (CCR7)low Intracellular/transcription factorEomesT-betintEffector memorySecretedGranzyme BIFN highI L- 2lowPerforinTNF highSurfaceCD44CD45RO CD62 LlowCD127 (IL7R )highCD197 (CCR7)

9 LowKLRG1 KRG1highIntracellular/transcription factorEomesintT-betintEffectorSecretedCC L3 (MIP-1 )CCL4 (MIP-1 )CCL5 (RANTES)Granzyme AGranzyme BGranzyme KIFN I L- 2 PerforinTNF SurfaceCD25hCD69hCD30CD122h CD137 (4-1BB)CD134 (OX40)CD223 (LAG-3)CD272 (BTLA)CD278 (ICOS)CD279 (PD-1)CD366 (TIM3)KLRG1 Intracellular/transcription factorT-betNa veSurfaceCD27CD45 RACD62 LCD127 (IL7R )CD197 (CCR7)Pan markersCD2CD3CD5CD7CD8CD25hCD27CD288 Central memorySecretedI L- 4lowIFN lowSurfaceCD44CD62L C D 12 7 ( I L-7 R ) CD197 (CCR7)high Intracellular/transcription factorbcl-6 EomesintT-betintEffector memorySecretedGranzyme BIFN I L- 2 PerforinTNF SurfaceCD44CD57 KLRG1 Intracellular/transcription factorEomesintT-betintBLIMP1 EffectorSecretedCCL3 (MIP-1 )CCL4 (MIP-1 )CCL5 (RANTES)Granzyme AGranzyme BIFN I L- 2 PerforinTNF SurfaceCD25hCD30CD44CD69hCD122hCD134 (OX40)CD137 (4-1BB)CD223 (LAG-3)CD272 (BTLA-4)CD278 (ICOS)CD279 (PD-1)CD