Transcription of 还原型谷胱甘肽（GSH）含量检测试剂盒说明书 Tel: 400-968 …
1 1 3 GSH BC1175 100T/96S 100 mL 1 4 C 20 mL 1 4 C 8 mL 1 4 C 10 mg 1 4 C 1 1mg 1mL 1mg/mL Glu Cys Gly -SH 5,5 - - - 2- 5,5 -dithiobis-2-nitrobenoic acid DTNB 2- -5- GSSG 2- -5- 412nm g/mL g/mL 2-3 / 96 1 PBS 2 1mL / 8000rpm 4 C 10min 4 C -80 C 10 2 4 C 600g 10 4 C Beijing Solarbio Science & Technology Co., LtdTel: 400-968 -6088 Fax: 010-56371281 2 3 8000g 10 4 C -80 C 10 4 C 600g 10 3 PBS 3 PBS 600g 10 4 C 10 8000g 10 4 C -80 C 10 3 500 PBS 2 PBS 600g 10 1mL 2-3 37 C 200W 3s 10s 30 8000g 10 4 C -80 C 10 1 30min 412nm 2 37 C 25 C 30min 3 20 L 140 L 40 L 2min 412nm A1 4 300 g/mL 200 g/mL 100 g/mL 50 g/mL 25 g/mL EP 20 L 140 L 40 L 2min 412nm A1 x y g/mL 5 20 L 140 L 40 L 2min 412nm A2 A= A2- A1 6 GSH A x y g/mL 1 GSH g/mg
2 Prot =y V V Cpr =y Cpr 2 GSH g/g =y V (V V W) =y W 3 GSH g/106 cell =y V (V V ) =y 4 GSH g/mL =2yV 1mL V 20 L= W g Cpr mg/mL 106 2 1 -80 C 2 3 GSH 3 3 4 5 [1] Fangzhou Chen,Ying Zhao,Huizhao Chen. MicroRNA-98 reduces amyloid -protein production and improves oxidative stress and mitochondrial dysfunction through the Notch signaling pathway via HEY2 in Alzheimer's disease mice. International Journal of Molecular 2018;91-102.( )[2] Ming Song,Fangfang Chen,Yihui Li,et al. rimetazidine restores the positive adaptation to exercise training by mitigating statin-induced skeletal muscle injury. Journal of Cachexia, Sarcopenia and Muscle. November 2017;( )[3] Hua Li,Lanying Wang,Yanping Luo.
3 Composition Analysis by UPLC-PDA-ESI ( )-HRMS and antioxidant Activity Using Saccharomyces cerevisiae Model of Herbal Teas and Green Teas from Hainan. Molecules. October 2018;( )[4] OuYang Q, Tao N, Zhang M. A damaged oxidative phosphorylation mechanism is involved in the antifungal activity of citral against Penicillium digitatum[J]. Frontiers in microbiology, 2018, 9: 239.[5] Chen Z Y, Wang Y T, Pan X B, et al. Amelioration of cold-induced oxidative stress by exogenous 24-epibrassinolide treatment in grapevine seedlings: Toward regulating the ascorbate glutathione cycle[J]. Scientia horticulturae, 2019, 244: 379-387. [6] GongSun X, Zhao Y Q, Jiang B, et al. Inhibition of MUC1 C regulates metabolism by AKT pathway in esophageal squamous cell carcinoma[J]. Journal of cellular physiology, 2019, 234(7): 12019-12028. [1] Alpert A J, Gilbert H F. Detection of oxidized and reduced glutathione with a recycling postcolumn reaction[J].
4 Analytical biochemistry, 1985, 144(2): 553-562.[2] Owens C W I, Belcher R V. A colorimetric micro-method for the determination of glutathione[J]. Biochemical Journal, 1965, 94(3): 705. BC1180/ BC1185 GSSG BC1190/ BC1195 GPX BC1150/ BC1155 TrxR BC1210/ BC1215 - GCL 4 3
