超氧化物歧化酶（SOD）活性检测试剂盒说明书 Tel: 400-968 …

1 BC0170 -- 1 4 SOD BC0170 50T/24S 60 mL 1 4 C 15 mL 1 4 C 160 L 1 4 C 11 mL 1 4 C 1 4 C 2 mL 1 4 C 1 2 4 C 3 SOD EC H2O2 O2 SOD H2O2 (O2-) O2- 560nm SOD O2- SOD 2-3 1mL / 1. 104 mL 500-1000:1 500 1mL 20% 200W 3s 10s 30 8000g 4 C 10min 2.

2 G mL 1:5-10 1mL 8000g 4 C 10min 3. Beijing Solarbio Science & Technology Co., LtdTel: 400-968 -6088 Fax: 010-56371281 BC0170 -- 2 4 1. 30min 560nm 2. 37 C 25 C 5min 3. EP L 1 2 9090-- 240240240240 6-6- 180180180180 480486570576 30303030 37 C 30min 1mL 560nm A A A1 A2 A =A -A A =A1 -A2 1 2 1~2 SOD 1 =( A - A ) A 100% 30-70% 50% 30% 70% 2 SOD 50% SOD 3 SOD 1 SOD (U/mL)=[ (1- ) V ] V F= (1- ) F 2 SOD A SOD (U/mg prot)

3 =[ 1- V ] V Cpr F= (1- ) Cpr FB SOD (U/g )=[ (1 - ) V ] (W V V ) F = (1- ) W FC SOD (U/104 cell)= [ (1- ) V ] (500 V V ) F= (1- ) FV V V 1mL Cpr mg/mL W g 500 500 F BC0170 -- 3 4 1 2 3 1 1mL A =A -A = A =A1 -A2 = =( A - A ) A 100%=72% SOD (U/g )= (1- ) W= U/g 2 1mL A = A -A = A =A1 -A2 = =( A - A ) A 100% = SOD (U/g )= (1- ) W= U/g 3 1000 1mL A =A -A = A =A1 -A2 = =( A - A ) A 100% = SOD (U/104 cell)= (1- ) V ] (1000 V V )= U/104 cell [1] Beibei Li,Yang Ding,Xiuli Tang,et al.

4 Effect of L-Arginine on Maintaining Storage Quality of the White Button Mushroom (Agaricus bisporus). Food and Bioprocess Technology. April 2019; 12:563-574.( )[2] Yanan Wang,Chengzhen Liang,Zhigang Meng,et al. Leveraging Atriplex hortensis choline monooxygenase to improve chilling tolerance in cotton. Environmental and Experimental Botany. June 2019; 162: 364-373. ( )[3] Yang Yang,Li Jing,Wei Cong,et al. Amelioration of nonalcoholic fatty liver disease by swertiamarin in fructose-fed mice. Phytomedicine. June 2019;59. ( )[4] Siyang Yu,Bo Dong,Zhenfei Fang,et of lncRNA AK139328 alleviates myocardial ischaemia/reperfusion injury in diabetic mice via modulating miR-204-3p and inhibiting autophagy.

5 Journal of Cellular and Molecular Medicine. July 2018;( )[5] Qilong Wang, Guosheng Xiao, Guoliang Chen,et al. Toxic effect of microcystin-LR on blood vessel development. Toxicological & Environmental Chemistry. February 2019;( ) [1] Spitz D R, Oberley L W. An assay for superoxide dismutase activity in mammalian tissue homogenates[J]. Analytical Biochemistry, 1989, 179(1):8-18.[2] Masayasu M, Hiroshi Y. A simplified assay method of superoxide dismutase activity for clinical use[J]. Clinica Chimica Acta, 1979, 92(3) -- 4 4 BC0190/BC0195 PPO BC0210/BC0215 PAL BC0200/BC0205 CAT BC0090/BC0095 POD