丙二醛（MDA）含量检测试剂盒说明书 Fax: 010-56371281 …

1 Beijing Solarbio Science & Technology Co., Ltd Tel: 400-968-6088. Fax: 010-56371281 . MDA .. BC0025. 100T/96S.. 110 mL 1 4 . 42 mL 1 4 . 2 4 . 12 mL 1 4 .. MDA 1 20mL 4 MDA . 70 .. MDA MDA . MDA thiobarbituric acid TBA . 3,5,5- -2,4- 532nm . MDA TBA . 450nm 532nm 600nm 532nm 450nm 532nm 450nm . 600nm MDA .. 2-3 .. / /96 / .. 1 500 1mL . 200W 3s 10s 30 8000g 4 10min .. BC0025 -- 1 4 . 2 1mL 8000g 4 10min .. 3 .. 1 / 30min . 2 . L . MDA 300 300. - 100. 100 - 100 100. 100 60min 10000g 10min . 200 L 96 450nm 532nm 600nm.

2 A450=A450 -A450 A532=A532 -A532 A600=A600 -A600 1-2 . MDA . A 96 . 1 MDA . 1 . MDA nmol/mg prot =( ( A532- A600) A450) V (Cpr V ). =5 ( ( A532- A600) A450) Cpr 2 . MDA nmol/g =( ( A532- A600) A450) V (W V V ). =5 ( ( A532 - A600) A450) W. 3 . MDA nmol/104 cell =( ( A532- A600) A450) V (500 V V ). = ( ( A532- A600) A450). 4 . MDA nmol/mL =( ( A532- A600) A450) V V . =5 ( ( A532 - A600) A450). V V mL Cpr mg/mL W . g 500 500 V 1mL . 2 MDA . 1 . MDA nmol/g =( ( A532- A600) A450) V (W V V ). =5 ( ( A532- A600) A450) W.

3 2 . BC0025 -- 2 4 . MDA nmol/mg prot =( ( A532- A600) A450) V (Cpr V ). =5 ( ( A532- A600) A450) Cpr V V mL Cpr mg/mL W . g V 1mL . B . 1 MDA . 1 . MDA nmol/mg prot =( ( A532- A600) A450) V (Cpr V ). =5 ( ( A532- A600) A450) Cpr 2 . MDA nmol/g =( ( A532- A600) A450) V (W V V ). =5 ( ( A532- A600) A450) W. 3 . MDA nmol/104 cell =( ( A532- A600) A450) V (500 V V ). = ( ( A532- A600) A450). 4 . MDA nmol/mL =( ( A532- A600) A450) V V . =5 ( ( A532 - A600) A450). V V mL Cpr mg/mL W . g 500 500 V 1mL . 2 MDA.

4 1 . MDA nmol/g =( ( A532- A600) A450) V (W V V ). =5 ( ( A532- A600) A450) W. 2 . MDA nmol/mg prot =( ( A532- A600) A450) V (Cpr V ). =5 ( ( A532- A600) A450) Cpr V V mL Cpr mg/mL W . g V 1mL .. 60min 90min MDA .. 1 96 A450=A450 -A450 = A532=A532 . -A532 = A600=A600 -A600 = . BC0025 -- 3 4 . MDA nmol/mL =5 ( ( A532- A600) A450)= nmol/mL . 2 500 hela 1mL 96 . A450=A450 -A450 = A532=A532 -A532 = A600=A600 . -A600 = . MDA nmol/104 cell = ( ( A532- A600) A450)= ( ( )- )= nmol/104 cell . [1] QianYi Peng,YiMin Wang,CaiXia Chen,et al.

5 Inhibiting the CD38/cADPR pathway protected rats against sepsis associated brain injury. Brain Research. January 2018;( . [2] Zhigang Chen , Qiaoling Yuan , Guangren Xu,et al. Effects of Quercetin on Proliferation and H2O2-Induced Apoptosis of Intestinal Porcine Enterocyte Cells. Molecules. 2018; ( ). [3] Huiwen Xiao, Yuan Li, Dan Luo,et al. Hydrogen-water ameliorates radiation-induced gastrointestinal toxicity via MyD88's effects on the gut microbiota. experimental and molecular medicine. January 2018;( ).)

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7 Journal of Plant Physiology. October 2018;( ). [7] Lijiao Gu, Hantao Wang, Hengling Wei,et al. Identification, Expression, and Functional Analysis of the Group IId WRKY Subfamily in Upland Cotton (Gossypium hirsutum L). Frontier in Immunology. November 2018;( ). [8] Ping Shao,Pei Wang,Ben Niu,et al. Environmental stress stability of pectin-stabilized resveratrol liposomes with different degree of esterification. International Journal of Biological Macromolecules. November 2018;( ).. [1]Spitz D R , Oberley L W.

8 An assay for superoxide dismutase activity in mammalian tissue homogenates[J]. Analytical Biochemistry,1989. [2]Masayasu M , Hiroshi Y . A simplified assay method of superoxide dismutase activity for clinical use[J]. Clinica Chimica Acta.. BC3590/BC3595 H2O2 . BC1090/BC1095 XOD . BC0690/BC0695 GOD . BC1270/BC1275 . BC1280/BC1285 DAO . BC1290/BC1295 . BC0025 -- 4 4.