Transcription of 甘油三酯（TG）含量检测试剂盒说明 ... - Solarbio
1 Beijing Solarbio Science & Technology Co., Ltd Tel: 400-968-6088. Fax: 010-56371281. TG .. BC0625. 100T/96S.. 160 mL 1 4 . 10 mL 1 4 . 20 mL 1 4 . 5 mL 1 4 . 15 mL 1 4 . 15 mL 1 4 . 1 4 .. 1 1:1 . 2 5 mL 1 mg/mL 4 .. TG . TG KOH TG . 420nm TG .. mg/mL. mg/mL. 2-3 .. / / /96 .. 1 g (mL) 1 5~10 1mL . 8000g 4 10min . 2 400-500 1mL 1min 20% 2s 1s 8000g 4 10min . 1 3 . 3 .. 1. / 30min 420 nm . 2. 65 .. L - 120 - TG L - - 120. L 495 375 375. L 75 75 75. 30s 3-5min 30s 3 . 30 L EP . 3 .. L 30 30 30. L 100 100 100. L 30 30 30. 65 3min . L 100 100 100. L 100 100 100. 65 15min . 200 L /96 420nm A A A . 1-2 . TG . 1.. TG mg/dL =C A -A A -A 100=100 A -A A -A.
2 C 1mg/mL 100 1dL=100mL . 2.. (1) . TG mg/ mg prot =C V A -A A -A Cpr V . = A -A A -A Cpr (2) . TG mg/ g =C V A -A A -A W= A -A A -A W. C 1mg/mL Cpr mg/mL W g V 1mL . 3.. TG mg/104 cell =C A -A A -A . = A -A A -A . C 1mg/mL 104 cell/mL . 2 3 .. 1.. 2.. 3.. 4. OD .. [1] Wei Hu,Rui Wei,Liyue Wang,et al. Correlations of MMP-1, MMP-3,and MMP-12 with the degree of atherosclerosis, plaque stability and cardiovascular and cerebrovascular events. Experimental and Therapeutic Medicine. 2018;( ). [2] Jieyong Xing,Yanshao Liu,Tao Chen. Correlations of chemokine CXCL16 and TNF- with coronary atherosclerotic heart disease. Experimental and Therapeutic Medicine.
3 November 2017;( ). [3] Zhenbin Xu,Xizhuang Bai. Strontium ranelate-induced anti-adipocytic effects are involved in negative regulation of autophagy in rat bone marrow mesenchymal stem cells. International Orthopaedics. October 2018;( ). [4] Chu X Y, Yang S Z, Zhu M Q, et al. Isorhapontigenin Improves Diabetes in Mice via Regulating the Activity and Stability of PPAR in Adipocytes[J]. Journal of Agricultural and Food Chemistry, 2020, 68(13): 3976-3985. [5] Li W, Li Y, Zhao Y, et al. The protective effects of aloperine against ox-LDL-induced endothelial dysfunction and inflammation in HUVECs[J]. Artificial Cells, Nanomedicine, and Biotechnology, 2020, 48(1): 107-115.
4 [1] Fletcher M J. A colorimetric method for estimating serum triglycerides[J]. clinica chimica Acta, 1968, 22(3): 393-397. [2] Hercules D M, Sheehan T L. Chemiluminescent determination of serum glycerol and triglycerides[J]. Analytical chemistry, 1978, 50(1): 22-25.. BC1890/BC1895 FC . BC0750/BC0755 ALDH . BC0410/BC0415 A ACC . BC1980/BC1985 TC . 3 3 . 4 3.
