Peptone, Bacteriological - HiMedia Labs

1 Please refer disclaimer , BacteriologicalRM001It contains high tryptophan content used as culture media ingredient in variety of media. It can also be used for commercialproduction of enzymes, vaccines, antibiotics,steroids and other And InterpretationPeptone, Bacteriological is prepared by enzymatic digestion of selected fresh meat. Being highly nutritious it supports goodgrowth of wide variety of microorganisms and can be used for identification of bacteria by performing various biochemical peptones confer nutritional benefit, especially at low dilution rates, for the recombinant cell lines it have been recently usedas medium additives for the production of a recombinant therapeutic protein in high density perfusion cultures of mammaliancells .Quality ControlAppearanceLight yellow to brownish yellow homogenous free flowing powder ,having Characteristic odour but not soluble in distilled/purified water, insoluble in w/v aqueous solution remains clear and neutral without any haziness after autoclaving at 15 lbs pressure (121 C) for of 2% w/v aqueous solution at 25 for Pathogens1.

2 In 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosa-Negative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. Negative in 10 gms ofsample6. Clostridia- Negative in 10 gms of sampleDegree of digestionAs per method specified in USP 32,NF26. a. Absence of undigested protein b. Presence of proteoses c. Presence oftryptophanNitrite testAs per method specified in USP 32,NF26 Negative:No development of pink or red ContentAs per method specified in USP 32,NF26 <=Total of 50 microorganisms or clumps in 10 consecutive TestingBacteriological tests are carried out as per USP 32,NF26 where respective medium is prepared by using peptone under for fermentable carbohydrateMedium :2% peptone w/phenol red broth w/durhams inoculation with test culture and incubation for 24 hours at35-37 C Acid production ,(Positive test) No acid production,(Negative test)Yeast & mould Count : <= 100 CFU/gram By plate method when incubated at 20-25 C for not less than 5 Count : <= 2000 CFU/gram By plate method when incubated at 30-35 C for not less than 3 Load.

3 Total Yeast and mould count (cfu/gm) Total aerobic microbial count (cfu/gm) Escherichia coli ATCC 25922 Streptococcus liquefaciensHiMedia LaboratoriesTechnical DataPlease refer disclaimer of H2 SMedium :1% peptone in inoculation with test culture and incubation for 24 hours at 35-37 C .The lead acetatetest paper shows brownish blackening (lead sulphide)Production of IndoleMedium : peptone in inoculation with test culture and incubation for 24 hours at 35-37 C. Appearance ofdistinct pink to red colour ring (Positive test). No formation of pink to red coloured ring (Negative test).Cultural responseCultural response observed after incubation at 35-37 C for 24 hours by using 2% peptone , sodium chloride and in water,pH ResponseOrganismGrowthCultural responseEscherichia coli ATCC25922 LuxuriantPseudomonas aeruginosaATCC 27853 LuxuriantEnterobacter aerogenesATCC 13048luxuriantSalmonella Typhi ATCC6539 LuxuriantStaphylococcus aureusATCC 25923luxuriantStreptomyces albus ATCC3004luxuriantStreptococcus pyogenesATCC 19615luxuriant w/beta haemolysis(With additionof sterile 5%sheep bloodto abovemedium, afteran incubation at35-37 C for gonorrhoeaeATCC 19424luxuriant w/beta haemolysis(With additionof sterile10% sheepblood to abovemedium heatedto 80-90 Cuntil bloodhas turnedto chocolatebrown andincubated in10% CO2atmosphere at35-37 C for 48hours).)

4 Escherichia coli ATCC 25922 Enterobacter aerogenes ATCC 13048 Salmonella Typhi ATCC 6539 Production of acetyl methyl carbinolMedium peptone and of dextrose in inoculation with test culture and incubation for 24 hours at35-37 C. Formation of pink colour (Positive test). No formation of pink colour (Negative test).Escherichia coli ATCC 25922 Enterobacter aerogenes ATCC 13048 HiMedia LaboratoriesTechnical DataDisclaimer :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time.

5 Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: and Shelf LifeStore below 30 C. Use before expiry date on the Nitrogen>= >= chloride<= on drying<= on ignition<= 15% Chemical Analysis