Polymer-to-Solvent Reference Table for GPC/SEC

1 Polymer-to-Solvent Reference Table for GPC/SECT echnical OverviewAuthorAdam Bivens, Agilent TechnologiesAbstractA comprehensive Reference on solvents for gel permeation chromatography, with a full list of tested solvent, polymer, and column combinations, and notes on best chemistry was formulated to be noninteractive to a wide variety of polymers dissolved in their respective solvents. Polymeric media allow for both wide pores and a single, consistent surface chemistry free of interactive silanols or metal centers found on other its 40-year history, Agilent has assembled a vast library of applications for GPC, which are summarized in Table GPC to yield valid data, the analytes must not stick to the surface of the particle, as this would cause the retention time to no longer refl ect just size, but also surface interaction overcome this challenge, Agilent provides three phase chemistries that minimize surface interaction in their respective solvents: PLgel A highly cross-linked polystyrene-divinylbenzene (PS-DVB) particle compatible with organic and many polar organic solvents.

2 Brochure: 5990-7994EN PolarGel A proprietary particle chemistry designed for polar samples dissolved in polar organics and water/ organic mixtures Brochure: 5990-7995EN PL aquagel-OH A hydrophilic particle chemistry compatible with water, high-salt buffers, and up to 50 % methanol Brochure: 5990-7995 ENIntroductionGel permeation chromatography (GPC), which is also referred to as size exclusion chromatography (SEC) or gel fi ltration chromatography (GFC), provides size-based separation by allowing analytes with small size in solution to diffuse into the pores of the media, while analytes that are too large to fi t into these pores are swept through the column by the solvent. Pore size and distribution can be manipulated to change the retention-to-size relationship and the range of separable sizes.

3 This retention-to-size relationship is established with calibration standards, but becomes inaccurate over time, and must be regularly 1. Chemical uorisopropanolBHTB utylated hydroxytolueneDMFD imethylformamideDMSOD imethylsulfoxideTCB1,2,4-Trichlorobenzen eNaTFAS odium trifl uoroacetateTEAT riethylamine3 ApplicationSolventPhaseTemperature( C)Publication number Acrylonitrile butadiene styrene (ABS) plasticDMF + % LiBrPolarGel505991-2517 ENAcrylonitrile butadiene styrene (ABS) plasticTHFPLgel405990-8495 ENAcrylonitrile styrene acrylate (ASA) plasticTHFPLgel255990-8601 ENAdipate polyestersTHFPLgel255990-8602 ENAlkanesTCBPLgel1005990-8494 ENAlkyd resinTHFPLgel255990-6845 ENAlkyl glyceridesTHFPLgel355991-1895 ENAlkyl glyceridesTHFPLgel255990-8322 ENAlkyl glyceridesTHFPLgel255990-8326 ENAlkylketene dimer (AKD)

4 THFPLgel255990-8402 ENASA PlasticTHFPLgel255990-8601 ENAsphalto-XylenePLgel605990-8493 ENAsphaltTHFPLgel505990-8494 ENBisphenol-A, quantifi cationTHFPLgel255990-8408 ENBitumino-XylenePLgel605990-8493 ENBituminTHFPLgel505990-8494 ENButyl rubberHexanePLgel255990-6866 ENCarbomer (PAA)Water + M NaNO3 + M NaH2PO4 adjusted to pH 7PL aquagel-OH255990-6920 ENCarboxymethyl cellulose (CMC)Water + M Na2SO4PL aquagel-OH255991-5792 ENCarboxymethyl cellulose (CMC)Water + M NaNO3 + M NaH2PO4 at pH 7PL aquagel-OH255991-5827 ENCelluloseDMAc + % LiBrPolarGel505991-2517 ENCelluloseDMSO + % LiBrPolarGel505991-2517 ENCellulose acetateDMAc + % LiClPLgel605991-5809 ENChitosanWater + M NaNO3 + M NaH2PO4 at pH 2PL aquagel-OH505991-5786 ENComb polymer, PolyacrylateTHFPLgel355991-2891 ENComb polymer, rubber, syntheticTHFPLgel355991-2891 ENCorn fl ourDMSO + % LiBrPLgel505991-5772 ENDextranWater + M NaH2PO4 + M NaCl at pH 7PL aquagel-OH255991-5791 ENDextranWater + M NaNO3 + M NaH2PO4PL aquagel-OH405991-5841 ENDiglycidyl ether bisphenol-A (DGEBA)

5 THFPLgel405991-5823 ENEmeraldine NMP + % LiBrPLgel805991-5814 ENEpoxy prepreg resinTHFPLgel255990-8218 ENEpoxy resinDMF + % LiBrPolarGel605991-5840 ENEpoxy resin oligomersTHFPLgel255990-8609 ENEpoxy resin oligomersTHFPLgel255990-8606 ENEpoxy resin oligomersTHFPLgel255990-8604 ENEpoxy resin, commercialTHFPLgel505990-8398 ENEpoxy resin, high MWTHFPLgel255990-6845 ENEthylene vinyl acetate (EVA)TCB + % BHTPLgel1605991-1690 ENTable 2. Agilent GPC ( C)Publication number Fatty acid methyl esters (FAME)THFPLgel355991-1895 ENFatty acid methyl esters (FAME)THFPLgel255990-8418 ENFlavonoids THFPLgel255991-0487 ENFluoroelastomerTHFPLgel405991-6624 ENGelatinWater + M NaNO3 + M NaH2PO4 at pH 7PL aquagel-OH255991-5796 ENGlyceridesTHFPLgel355991-1895 ENGlyceridesTHFPLgel255990-8322 ENGlyceridesTHFPLgel255990-8326 ENGum arabicWater + M NaH2PO4 + M NaNO3 at pH 7PL aquagel-OH255991-5790 ENHyaluronic acidWater + M NaNO3 + M NaH2PO4 at pH 7PL aquagel-OH255991-5787 ENHydrocarbons, linearTCBPLgel1455990-6971 ENHydrocarbons, linearTCBPLgel1005990-8494 ENHydrocarbons, long chainTCBPLgel1455990-6971 ENHydrocarbons, long chainTCBPLgel1005990-8494 ENHydrocarbons.

6 Short chainTCBPLgel1005990-8494 ENHydroxyethyl celluloseDMF + % LiBrPLgel505991-4299 ENHydroxyethyl celluloseWater + M NaH2PO4 + M NaCl at pH 7PL aquagel-OH505991-4299 ENHydroxyethyl cellulose, Modifi edWater + M NaH2PO4 + M NaCl at pH 7PL aquagel-OH505991-5793 ENIsocyanate prepolymersDichloromethanePLgel255990-79 84 ENIsocyanate prepolymersTHFPLgel255990-8219 ENIsocyanate resinTHFPLgel255990-6845 ENLigninDMF + % LiBrPolarGel505991-5829 ENLigninDMSO + % LiBrPolarGel505991-5765 ENMaltodextrins, in starchWater + M NaNO3 + M NaH2PO4, adjusted to pH 7 PL aquagel-OH255991-2029 ENMelamine resinDMAC + 1 % LiBrPolarGel505991-5830 ENMelamine resinDMSO + % LiBrPolarGel505990-6845 ENMelamine-formaldehyde resinDMFPLgel805990-8419 ENMethyl celluloseWater + M NaH2PO4 + M NaCl at pH 7PL aquagel-OH505991-2519 ENNatural rubber, vulcanizedToluenePLgel255990-8407 ENNovalacDMFPLgel505990-8486 ENNovalacTHFPLgel255990-7981 ENNovalac resinDMF + % LiBrPolarGel505991-5767 ENNovalac resinDMSO + % LiBrPolarGel505991-5766 ENNylonHFIP + 20 mM NaTFAHFIPgel405991-0485 ENNylonm-CresolPLgel1005991-0485 ENNylon 6, low MWTHFPLgel255990-8423 ENOdorants, essential oils, acid estersTHFPLgel255990-7982 ENOil, lubricant.

7 Certifi ed 3100 MWTHFPLgel305991-4312 ENOil, lubricant, petroleum jellyTHFPLgel255991-5820 ENOligopinTHFPLgel255991-0487EN5 ApplicationSolventPhaseTemperature( C)Publication number OligosaccharidesNMPPLgel805990-8489 ENOligosaccharides, xyloseWaterPL aquagel-OH255990-8399 ENPaint, resin, commercialTHFPLgel255990-6845 ENPectinWater + M NaNO3 + M NaH2PO4, adjusted to pH 7 PL aquagel-OH505991-4086 ENPectinWater + M NaNO3 + M NaH2PO4 at pH 7PL aquagel-OH255991-5794 ENPetroleum jellyTHFPLgel255991-5820 ENPhenol distillate residueAcetonePLgel255990-8421 ENPhenol-formaldehyde resinDMF + % LiBrPolarGel505991-5767 ENPhenol-formaldehyde resinTHFPLgel255990-7983 ENPhthalates, dialkyl, PlasticizerTHFPLgel255990-8324 ENPolacrylate, combTHFPLgel355991-2891 ENPoloxamerDMF + LiBrPLgel705990-8296 ENPoly(2-vinyl pyridine)

8 Water + M NaNO3 + M NaH2PO4 at pH 7PL aquagel-OH255991-5780 ENPoly(2-vinyl pyridine) Water + M NaNO3 + M NaH2PO4 at pH 3PL aquagel-OH255991-5881 ENPoly(4-bromostyrene)THFPLgel255991-583 9 ENPoly(acrylates)DMAc + % LiBrPolarGel505991-2517 ENPoly(acrylates)DMF + % LiBrPolarGel505991-2517 ENPolyacrylonitrile (PAN)DMF + % LiBrPolarGel505991-2517 ENPoly(aminostyrene - vinyl pyrrolidone)Water + M NaNO3 + M NaH2PO4, adjusted to pH 7 PL aquagel-OH255991-5781 ENpoly(ester-imide)THFPLgel255990-6845 ENPoly(ethylene oxide), high MWDMF + % LiBrPolarGel505991-2517 ENpoly(ethylene-vinyl acetate) (PEVA)TCB + % BHTPLgel1605991-1690 ENPoly(isobornyl methacrylate) (IBMA)THFPLgel255991-5819 ENpoly(lactic-co-glycolic acid) (PLGA)ChloroformPLgel255990-8401 ENpoly(lactic-co-glycolic acid) (PLGA)THFPLgel405991-5776 ENPoly(methyl vinyl ether-maleic acid)Water + M NaNO3 + M NaH2PO4, adjusted to pH 7 or pH 9 as appropriatePL aquagel-OH25SI-01965 Poly(methyl vinyl ether-maleic acid) alkyl esters Water + M NaNO3 + M NaH2PO4, adjusted to pH 7 or pH 9, as appropriatePL aquagel-OH255991-5799 ENPoly(n-isopropylacrylamide) (PNIPAM)THF + 5 % TEAPLgel405991-5834 ENPoly(styrene-isoprene) Block CopolymerTHFPLgel255990-8228 ENPoly(styrene butadiene) Copolymer (SBR)THFPLgel405991-5771 ENPoly(vinyl chloride) (PVC)THFPLgel255991-5813 ENPoly(vinylidene fl uoride) (PVDF)

9 DMSOPLgel955990-8328 ENPolyacrylamide (PAM)Water + M Na2SO4 at pH 3PL aquagel-OH255991-5789 ENPolyacrylamide (PAM)Water + M NaNO3 + M NaH2PO4 at pH 7PL aquagel-OH405991-5773 ENPolyacrylic acid (PAA)Water + M NaNO3 + M NaH2PO4, adjusted to pH 7 PL aquagel-OH255991-5783 ENPoly-alpha-olefi n (PAO)TCB + % BHTPLgel1605990-6971 ENPolyamideHFIP + 20 mM NaTFAHFIPgel405990-7978 ENPolyanilineNMP + % LiBrPLgel805991-5814 ENPolyanion, acrylic acid, sodium saltWater + M NaNO3 + M NaH2PO4, adjusted to pH 7 PL aquagel-OH255991-5783EN6 ApplicationSolventPhaseTemperature( C)Publication number Polyanion, polyacrylamideWater + M Na2SO4 at pH 3PL aquagel-OH255991-5789 ENPolyanion, polystyrene sulfonate80 % [Water + M NaNO3 + M NaH2PO4 at pH 9] + 20 % MethanolPL aquagel-OH255991-5778 ENPolybromostyreneTHFPLgel255991-5839 ENPolybutadieneTHFPLgel405991-5833 ENPolybutylene Terephthalate (PBT)HFIP + 20 mM NaTFAHFIPgel405990-8220 ENPolybutyrate resinTHFPLgel255990-8602 ENPolycaprolactamHFIP + 20 mM NaTFAHFIPgel405991-0485 ENPolycaprolactamm-CresolPLgel1005991-04 85 ENPolycaprolactam, low MWTHFPLgel255990-8423 ENPolycarbonateDichloromethanePLgel25599 0-7894 ENPolycarbonateTHFPLgel405991-5822 ENPolycation, poly(2-vinyl pyridine)

10 Water + M NaNO3 + M NaH2PO4 at pH 7PL aquagel-OH255991-5780 ENPolycation, poly(2-vinyl pyridine) Water + M NaNO3 + M NaH2PO4 at pH 3PL aquagel-OH255991-5881 ENPolycation, poly(aminostyrene-vinyl pyrrolidone) Water + M NaNO3 + M NaH2PO4, adjusted to pH 7 PL aquagel-OH255991-5781 ENPolycation, polyacrylamideWater + M Na2SO4 at pH 3PL aquagel-OH255991-5789 ENPolydimethyl siloxane (PDMS)ToluenePLgel255990-7893 ENPolyesterTHFPLgel505991-5775 ENPolyester, adipate resinTHFPLgel255990-8602 ENPolyester, polyol resinTHFPLgel255990-8325 ENPolyether ethyl ketone (PEEK)80 % Chloroform + 20 % Dichloroacetic acidPLgel255990-8422 ENPolyether sulfoneDMF + % LiBrPLgel605991-5811 ENPolyetherimide (PEI)DMF + % LiBrPLgel605990-8222 ENPolyethyleneTCB + 15 % BHTPLgel1605991-5826 ENPolyethyleneTCB + 15 % BHTPLgel1605990-6971 ENPolyethylene glycol (PEG)DMF + % LiBrPLgel505991-5825 ENPolyethylene glycol (PEG)WaterPL aquagel-OH255990-6920 ENPolyethylene glycol (PEG), branchedDMF + % LiBrPLgel505991-5825 ENPolyethylene glycol, star70 % [Water + M NaNO3 + M NaH2PO4] + 30 % methanol PL aquag