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QIAGEN DNA Purification - ualberta.ca
four stages: 1. Disruption 2. Lysis 3. Removal of proteins and contaminants 4. Recovery of DNA In some methods, stages 1 and 2 are combined. Preparation of crude lysates An easy technique for isolation of genomic DNA is to incubate cell lysates at …
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Modern Raman Spectroscopy: Has the sleeping …
www.chem.ualberta.caStated differently: Has Raman spectroscopy made the transition from research tool to widely used routine analytical technique? (Note: …
SN2 , SN1 , E2 , & E1: Substitution and Elimination Reactions
www.chem.ualberta.caIntro Chem Handouts Substitution & Elimination Reactions Page 1 of 3 SN2 , SN1 , E2 , & E1: Substitution and Elimination Reactions l Nucleophilic Substitution Reactions - SN2 Reaction:
18- Electron Rule.
www.chem.ualberta.ca18 Electron Rule cont’d Example 1. [Co(NH 3) 6] +3 Oxidation state of Co? Electron configuration of Co? Electrons from Ligands? Electrons from Co? Total electrons? Example 2. [Fe(CO) 5] Oxidation state of Fe? Electron configuration of Fe? Electrons from Ligands? Electrons from Fe? Total electrons? What can the EAN rule tell us about [Fe(CO) 5]?
Table of Isotopic Masses and Natural Abundances
www.chem.ualberta.ca11 B 11.009305 80.1 6 Carbon 12 C 12.000000 98.93 13 C 13.003355 1.07 14 C 14.003242 * 7 Nitrogen 14 N 14.003074 99.632 15 N 15.000109 0.368 8 Oxygen 16 O 15.994915 99.757 17 O 16.999132 0.038 18 O 17.999160 0.205 9 Fluorine 19 F 18.998403 100 10 Neon 20 Ne 19.992440 90.48 21 Ne 20.993847 0.27 22 Ne 21.991386 9.25
Table of Isotopic Masses and Natural Abundances
www.chem.ualberta.caZ Name Symbol Mass of Atom (u) % Abundance 57 Fe 56.935399 2.119 58 Fe 57.933280 0.282 27 Cobalt 59 Co 58.933200 100 28 Nickel 58 Ni 57.935348 68.0769 60 Ni 59.930791 26.2231 61 Ni 60.931060 1.1399 62 Ni 61.928349 3.6345 64 Ni 63.927970 0.9256 29 Copper 63 Cu 62.929601 69.17 65 Cu 64.927794 30.83 30 Zinc 64 Zn 63.929147 48.63 66 Zn 65.926037 27.90
Nomenclature of substituted benzene rings
www.chem.ualberta.caA polychlorinated biphenyl (PCB) If the Cl’s were Br’s this would be a polybrominated biphenyl (PBB) CH 3CH 3 Br 2 hv CH 3CH 2Br + HBr. In the case of alkanes such as ethane or cyclohexane, light (hv) or heat are required for the bromination to occur (this is a substitution). For benzene, there is still no reaction
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Hydrogen Carriers for Bulk Storage and Transport of …
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Presentation - Manufacturing process of biologics
www.ema.europa.eu• Description: o Applicant’s commitment for the manufacture of the drug substance. o Manufacturing process and process controls. o Typically starts with a vial(s) of the cell bank, and includes cell culture, harvest(s), purification and modification reactions, filling, storage and shipping conditions. • Batch(es) and scale definition: o Explanation of the batch numbering …