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RT-qPCR guidelines - Real-time PCR Research and ...
For SYBR Green I, Cq of the NTC E Calibration curves with slope and y intercept E PCR efficiency calculated from slope E CIs for PCR efficiency or SE D r2 of calibration curve E Linear dynamic range E Cq variation at LOD E CIs throughout range D Evidence for LOD E If multiplex, efficiency and LOD of each assay E
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