Schwarz Differential Medium - himedialabs.com

1 Please refer disclaimer Differential MediumIngredientsGms / (Glucose) pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 1000 ml purified / distilled water. Heat to boiling with constant stirring for 15 minutes. DO NOT AUTOCLAVE. Cool to 45-50 C and pour into sterile plates. Efficacy of the plates can be improved by incubating them to 30 C for 18 hours before : Basic fuchsin is a potential carcinogen and care should be taken to avoid inhalation of the powdered dye andcontamination of the And InterpretationSchwarz Differential Medium is recommended for use in the brewing industry for the differentiation of brewing yeasts from wild yeasts (1,4).

2 Malt extract, peptone and yeast extract provide necessary nutrients to support the growth of yeasts. Dextrose is the suitable carbohydrate for the growth of yeasts. Sodium sulphite and basic fuchsin inhibit the gram-positive prepared plates darken during incubation. Wild yeasts form pink colonies which may be smooth, mucoid or wrinkled. brewing yeasts forms a thin haze of micro colonies which blend with the colour of the Use:Used in the brewing industry for the differentiation of brewing yeasts from wild **Type of specimen Brewery sampleFor brewery samples follow appropriate techniques for handling specimens as per established guidelines (2,3).

3 After use, contaminated materials must be sterilized by autoclaving before discarding. Specimen Collection and Handling: Warning and Precautions :Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data to nutritional variation, certain strains may show less growth. expiry period Performance and EvaluationPerformance of the Medium is expected when used as per the direction on the label within thewhen stored at recommended LaboratoriesTechnical DataCandida albicans ATCC10231 (00054*)50-100luxuriant>=50%white to lightpink raisedcoloniesCandida kruisei ATCC2440850-100luxuriant>=50%pink, rough, flatcoloniesSaccharomyces cerevisiae ATCC 9763 (00058*)50-100luxuriant>=50%pink coloniesReference1.

4 A. van der Aa K hle, L. Jespersen, Detection and identification of wild yeasts in lager breweries, Int. J. of Food Microbiol., Vol. 43, 3, p 205-213 (1998).2. Isenberg, Clinical Microbiology Procedures Handbook 2nd Jorgensen, , Pfaller, , Carroll, , Funke, G., Landry, , Richter, and Warnock., (2015) Manual of Clinical Microbiology, 11th Edition. Vol. L. Jespersen, M. Jakobsen, Specific spoilage organisms in breweries and laboratory media for their detection, Int. J. of Food Microbiol., Vol. 33, 1, p 139-155 (1996).5. T. De k, Beuchat, Handbook of food spoilage yeasts, 2nd Edition (2007)Revision : 02 / 2019 Disclaimer :User must ensure suitability of the product(s) in their application prior to use.

5 Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any ControlAppearancePinkish purple to Purple coloured homogeneous free flowing powderGellingFirm, comparable with agar and Clarity of prepared mediumLight pink coloured clear to slightly opalescent gel forms in Petri platesReactionReaction of aqueous solution at 25 C.

6 PH : ResponseCultural response observed at 30 C for upto 4 days. (colour of plates darkens during incubation).OrganismInoculum(CFU)GrowthR ecoveryColour ofColonyKey : *Corresponding WDCM and Shelf Life Store between 10-30 C in a tightly closed container and the prepared Medium at 20-30 C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use.

7 Product performance is best if used within stated expiry period. User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3). DisposalHiMedia Laboratories Pvt. Ltd. : 23, Vadhani , LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Website.