Transcription of Solobacterium moorei 1
1 Solobacterium moorei 1 1) 1) 1) 1) 1) 1) 2)1) 2) ! ! $% & 20(6#23) + & 20(8#18) , - &' / , Solobacterium moorei . 0 3 5 7 9 : 4; < 7=8 :> =? 360@ , 40A ?@ B C C DEFE F GG 9 H J3 H K L 3 IJ IJ2)Q &' . V W5 / Prevotella melaninogenica $L 3 / Z RapID ANAII, API20A [ Eggerthella lenta O 3 $L ^ `ab d fghi[ E. lenta E j k J 33? S 5 3 l 16S rRNA V nWXo 3pq Solobacterium mooreinY ,J3 3 t[ Z ^ b S. moorei \Q w 3? 5 ` , y za , E. lenta bc d e{ k a-glucosidase E. lenta , f S. moorei / , g ~h5 ij , k 3 ) Q[7 m $ n V Z e{ o p.]]]]}}
2 Pq 3 Key words: Solobacterium moorei V 16S rRNA,Eggerthella lenta Solobacterium moorei 2000( Kageyama 1) .J s3 . 3- &' / , S. moorei Solobacterium u , vw1) Q [ yz2), 3) $L S. moorei IJ $L 3 b { 3 4 6) 3 =? ? IJ S. moorei $L 3Q 3 5 7 60@ ~ a 9 : :> < 7=8 H K . 40A ; 72 mmHg ; 44 mmHg . 75/$ ,J3 H K ^ y1 W5 WBC 103/ml W CRP mg/dl e{ 3 a a [ = =? 3 ~ 2002( 9 : F L 3 2005( ? 3?9 L l Q j 3?H 3 2006(11#< 7= :> =? Q 7 V 3 l Q , 3 L 3 2007(1#31)40A ?]]}}
3 @ B C C ( 090 8666) t 6 2 1 TEL: 0157 24 3115 1258 FAX: 0157 22 3339E-mail: t Vol. 18 No. 4 2008. ) 200816236 ! # &' () + pazufloxacin1,000 mg/day 7 , micafungin 50 mg/day 16 , -. /0 7 12cefo-zopran 1 g/day 7 , 14 34cefpirome 1 g/day 6 &' 8 1 7 :3 8 <3= ( > @ B 0 0 ! " C # $D E @& 1 40 * H4 CRP @ 9 23 mg/dl J &K . L /'01 ) &K M @ 2 16 2O15 P3 1. 4$ :3 &' 8 S126& UVW /0 &' () 92F B9Y [\ ^ 93F B9Y [\ ^ > @ :BD 9> &' = BACTEC 9120 :BD a9 2 B9Y [\ ^ b ? B9Y [\ ^ 7 J0 c E 1 2.]]]]
4 1) BACTEC9120J? 93F B9Y [\ ^ B' d LE0# e GHI: f ghi K Bartholomew & Mitt-wer1 k M K L lm J) 2 ghi? N0 O2 P oQp 3 @ 1 1 oQ K sh 1 t ( 2) 2 ? 93F B9Y [\ ^ B' uU v GH + w y II 5~ &' X TSA :BD BYyU Y X BYCHO :BD BTB X BTB :BD ABHK X ABHK Y NV ABHK X NVABHK Y :3 BBE X BBE :BD a9 L ^y ( Q L TSA BYCHO 35 7~CO2 BTB 35 B LABHK NVABHK B VW y9 lm [ 9>L35 B# JH 3) ^y LABHK c ghi? N0 >L Sh t s lm :3 [ ^ RID Zyme IND-S lms [ ( _ V RapID ANAII i API 20A : sw :3 CRYSTALANR :BD a9 `# 0U #_ 16S rRNA a bc d 3 ghi N0 >L Sh RapID ANAII 9>L0U _ 4) Pg0 MIC h_ h Y lm ^ h lm a9 35 48 , B MIC _ ) 5)S.]]]]]
5 moorei Eggerthella lentaATCC ! Pg0 _ ( RapIDANAII API 20AJ 0Uj L 1 l H E. lenta 0 ATCC43055 m 0 p uU _ V >L Pg0 ) s0 ABHKJ48 , B 9> 0p ghi K Bartholomew & Mittwer1 34)> _ V RapID ANAII API20A:3 CRYSTAL ANR a9 0' t Q:3 _ , u V v L ( E. lenta z1 &' 8 SPeripheral bloodBlood chemistryRBC 106 g/dlAST59 ~ALT55 IU/LLD160 IU/LWBC 103/mlTotal-Bill ~ mg/dlLym ~ mg/dlMono ~Na130 mEq/LEosino ~ mEq/LBaso ~Cl87 mEq/LPlt272 103 mg/dlSolobacterium moorei6& 1{ Solobacterium moorei6& 1{ : }m Vol. 18 No. 4 3. 1) !"# $ ( * 0 1 2 5 067 29 : 9 $< 9 $(> 1 A1 9 $ D F !)}
6 9 G 2) TSA BYCHO BTB H 6K LM 48 NOP 9 $< 9 $ 2Q S ( 1 9 $ ABHK XY 1mm Z ! [ \ ] S < A2 9 $ ABHK<NVABHK ^! H 2_ ( BBE 6K `a2Q b$ de ! gh 1 $2 < i 3) 9 $ * kl d o kl d el d 9 G RapID ANAII S u e (020041, v> Eggerthella lenta(Wade et al.,1999)7)< v 1 API20A E. lenta S u e 00000002, v> <6 ( CRYSTAL ANR Atopobium minu-tum S u e 2233470000 v> < v 1 CRYSTAL ANR v> (z | 9 (G RapID ANAII<API 20A v~ K E. lenta< * kl d( 9 G\ < ( 9 G\< 6E. lenta 90< ( 1 1 ~ K $Q v 6K # \$Q v Q $ 16 SrRNA (S. moorei (JCM10645T) 1< i \ <( K A1 de !))))))))
7 A 92 : 9 $ $ ( $2 ( G\ A N # 1 9 $ G\ Bartholomew & Mittwer 1,000 A2 Solobacterium moorei S g LM 48NO ABHK % & % & * Vol. 18 No. 4 S. moorei !" # % &Q +,-! 1 2 BBE34 6 : ;+,<= ,> Prevotella melaninogenica?@# % RapIDANAII B !" F G # # % 4) S. moorei ! M O,PQR T2 U W# M MICXY chloramphenicol?4mg/ml sparfloxacin? [X U ? M W#] ` MICXYa cd e B cefozopran MICX ` fX U P. melaninogenica M O, g sparfloxacin?2mg/ml h, U ? j M k O,Ya 5)S. moorei Eggerthella lentaATCC m2 6n!
8 O qY E. lenta?r&< , w-! q %n x S. moorei yz! { , U , -!Ya |3 # ; , RapID ANAIIY S. moorei ?arginine,serine 6 a-glucosidase , U E. lenta arginine serine? ,Ya ? a-gluco-sidase +,Ya RapID ANAII yz! E. lentaATCC < R 1), 6), 8) % nS. moorei ,> T3 U API 20A S. moorei E. lenta Y F < E. lentaATCC 6n PQY , < E. lenta ,> U CRYSTAL ANRY S. moorei E. lentaATCC Y , ?F % arginine, serine, glycine, ala-T2yz % Solobacterium moorei M O,PQR Antimicrobial agentMIC(mg/ml)Ampicillin G acid |3 Solobacterium moorei (A) Eggerthella lentaATCC43055 (B) m2 S. moorei ! & ? w-> {, U &Q E. lenta r&<-> U n Bartholomew & Mittwer 1,000 Solobacterium moorei 1 Solobacterium moorei 1 Vol.}}
9 18 No. 4 , pyroglutamic acid, lysine, methionine, phen-ylalanine, leucine !" #$ 2 ' #$ ) *+ ,#- .E. lenta/ 1 2 Q 4 56 . ! . 8 9 1 6 2 3 => ? 29 /. A D ' #$Eubacte-rium !E ! G AH I # /E K 2 # N O#$ 4Q 2 A R E. lenta !S E T WX 1$ => ?2 A/#4 89)R9 / ' . [\2 A/]^ 6 E. lenta `ab Ec b /2 #10) !" chloramphenicol/sparfloxa-cin MIC f"gh A . `ab jEc b j $ $k MIC $56 mn % . E. lenta $ q & !?/ $Xr /. A sg 4 . tuv wx y $ z{9E ' ? j} a #$R) E. lenta/ + A / 4 ? . A! 4 . 4/ 0> v *+ Solobacterium moorei / )!
10 ' E 3 $# # 21$ 4 uv . ! A / $ . A - A! 6 . 1 / . A2 2 4 . # ? 2 $ uv .S. moorei A# A / . # /]^ . S. moorei /E. lenta ? 8 u9 A 1 API 20A/CRYSTAL ANR. $ I . K :! G A / . 1 E. lentaI " x2 API 20A.. 1 *+ ' m $ # # / ^ 2! ) ?. # A x/- 2 . A / 3 !"/Eggerthella lentaATCC43055 RapID ANAII. / R => ? 8 Biochemical reaction or enzymeResultsPublishedS. mooreistrainaIsolateE. lentaATCC43055 Urease p-Nitrophenyl-b,9-disaccharide (b-Disaccharidase) NTp-Nitrophenyl-a,A-arabinoside (a-Arabinosidase) o-Nitrophenyl-b,9-galactoside (b-Galactosidase) p-Nitrophenyl-a,9-glucoside (a-Glucosidase) p-Nitrophenyl-b,9-glucoside (b-Glucosidase) p-Nitrophenyl-a,9-galactoside (a-Galactosidase) p-Nitrophenyl-a,A-fucoside (a-Fucosidase) p-Nitrophenyl-n-acetyl-b,9-glucosaminide (N-Acetyl-glucosaminidase) p-Nitrophenylphosphate NTLeucyl-glycine-b-naphthylamide NTGlicine-b-naphthylamide NTProline-b-naphthylamide (Proline arylamidase) Phenylalanine-b-naphthylamide NTArgnine-b-naphthylamide (Arginine dehydrogenase)
