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SPECTROPHOTOMETRY (QUANTIFICATION OF NUCLEIC ACIDS
Spectrophometric measurements •The A260/280 ratio shows the purity of the sample analysed. •Pure DNA sample gives a 260:280 ratio of ˷1.8 and for pure RNA the ratio is ˷ 2. •A260/230 ratio indicates the presence of organic contaminants, such as (but not limited to): phenol, TRIzol, chaotropic salts and other aromatic
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Beginning Perl for Bioinformatics - CGIAR
hpc.ilri.cgiar.orgIT-SC $ Beginning Perl for Bioinformatics James Tisdall Publisher: O'Reilly First Edition October 2001 ISBN: 0-596-00080-4, 384 pages This book shows biologists with little or no programming
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260/280 and 260/230 Ratios - CGIAR
hpc.ilri.cgiar.orgSpectrophotometer to results obtained using other spectrophotometers, it is important to ensure that the pH of an undiluted sam-ple measured on our instruments is at the same pH and ionic strength as the diluted sample measured on the conventional spec-trophotometer.
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