Streptococcus dysgalactiae - suigi.jp

1 , ,7-12, 22 . Bull. Kagoshima Pref. Fish. Tec. Dev. Cen., ,7-12,2010.. ( Streptococcus dysgalactiae . 1) 2). 1) 2) . A new type Streptococcus infection in Cultured amberjack Seriola dumerili and yellowtail Seriola quinqueradiata ( Streptococcus dysgalactiae Infection). Tatumu Hirae Takuya Murase Harunao Nibe and Terutoyo Yoshida Abstract In 2002, mortalities due to infection with an unknown Streptococcus sp. occurred in cultured amberjack Seriola dumerili and yellowtail S. quinqueradiata. This infection had high clinical similarity to Lactococcus garvieae infection. Using Lancefield grouping and genetic investigation, the unknown Streptococcus sp.

2 Was identified as Lancefield group C Streptococcus dysgalactiae ( GCS) . For diseased fish, bacteria should be isolated from the necrosis site of the caudal peduncle since bacterial isolation from the brain is difficult. GCS. infection in fish is characterized by severe necrosis of the caudal peduncle with little exophthalmos. Genetic characterization by biased sinusoidal field gel electrophoresis ( BSFGE ) revealed different genotypes between fish and animal GCS isolates. The partial sequence of the 16S-23S rDNA intergenic spacer region of all the tested fish isolates differed from mammalian isolates in one or two nucleotides.

3 Although GCS was resistant to drugs such as oxytetracycline ( OTC) , resistance to erythromycin (EM ), lincomycin ( LCM ) , and aminobenzylpenicillin ( ABPC) was not observed. Until 2002, the use of drugs in fish farms in Kagoshima Prefecture had decreased because of the prevalent practice of L. garvieae vaccination. However, drug use has been increasing since 2003 due to the fish mortality caused by GCS infection. 2002 .. Lancefie . ld C . Streptococcus dysgalactiae 1) .. Lactococcus garvieae .. ) .. 2003 .. 1,000 . -7- . START:5mHz END: 160mHz ApaI . DC48V AC300V AC.

4 NaCl START:5mHz END: 260mHz .. 37 24 . PASTOR . EX STREP C . C . L garvieae .. 1 3 .. (S dysgalactiae ) 4 . ( ) 5 S dysgalactiae . 4 m L garviae . 6 4 .. 1,000 . L garviae 2 . S dysgalactiae 10 .. 6 . 2002 2006 S dysgalactiae 196 C . C . SIGMA . Wako Wako 2 . Wako 171 . Wako 6 107 . MIC (1981) .. 7 .. ATTO GENOFIELD . S. ma I 30 Apa I 37 8 . 12 19 SmaI . DC48V AC300V AC 1 . -8- , ,7-12, 22 . Bull. Kagoshima Pref. Fish. Tec. Dev. Cen., ,7-12,2010. 2) .. 9 10) . 1995 1998 . 2 3 1998 200 .. 2002 2003 .. 2003 2003 . 11 . 12. 4 S dysgalactiae 5 L garviae 10. 8.. 20,000.

5 6. 4. 2. 0. 1 4 7 10 1 4 7 10 1 4 7 10 1 4 7 10 1 4 7 10 1 4 7 10 1 4 7 10. 2003 2004 2005 2006 2007 2008 2009 . 6 9 . 100 10. 8. 80.. 6.. 60. 4. 40 2. 0. 20 1 4 7 10 1 4 7 10 1 4 7 10 1 4 7 10 1 4 7 10 1 4 7 10 1 4 7 10. 2003 2004 2005 2006 2007 2008 2009 . 0.. 10 .. 400 1,400. 7 ( < ) 350 1,200. 300.. 1,000.. 250. 200. 800. 600. 150. 400. 2009 12 100. 50 200. 8 . 0 0. 94. 95. 96. 97. 98. 99. 00. 01. 02. 03. 04. 05. 06. 07. 08. 09.. 19. 19. 19. 20. 20. 20. 20. 19. 19. 19. 20. 20. 20. 20. 20. 20. 11 .. 2002 2006 , S dysgalactiae 196. MIC .. 8 EM /ml ABPC . -9- . /ml LCM.

6 /ml 06 2 . 25 /ml OTC 13 S I . 25 /ml 50 /ml FF No1 13 14 . /ml KM /ml 15 18 . 95 . S1 S8 . EM (ABPC) 14 Apa I . LCM 2000 2002 Sma I . 2003 No1 13 14 . 12 15 18 . Sma I 95 . 1 02 04 S dysgalactiae MIC Apa I 122 . A1 A20 20 Sma . Apa I . 4 S I . S1 95 82 . S7 S8 .. 2 05 S dysgalactiae MIC 5 Apa I . A1 29 20 . A A4 21 20 . A1 A2. A8 . A17 A20 .. 3 06 S dysgalactiae MIC . 13 Sma . 12 . 14 Apa I . - 10 - , ,7-12, 22 . Bull. Kagoshima Pref. Fish. Tec. Dev. Cen., ,7-12,2010.. 4 Sma I .. EM . ABPC .. 5 Apa I 2006. LCM .. S dysgalactiae .. 2002 8 .. C .. 2004 .. - 11 - .. 1).

7 16 . 2004 169-185. 2) . 2003 12 41. 3) . 2004 6 52-55. 4)R Nomoto L I Munasinghe D-H Jin Y Shimahara, H Yasuda, A Nakamura N Misawa, T Itami and T. Yoshida Lancefield group C Streptococcus dysgalact- iae infection responsible for fish mortalities in Japan . Journal of Fish Diseases 2004 27,679-686. 5)R Nomoto N Unose, Y Shimahara, A Nakamura, T. Hirae, K Maebuchi, S Harada, N Misawa, T Itami, H. Kagawa, T Yoshida Characterization of Lancefield group C Streptococcus dysgalactiae isolated from farmed fish Journal of Fish Diseases 2006 29, 673- 682. 6) .. 16 . 2004 189-198.

8 7) .. 17 . 2005 145-154. - 12.