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7 7) CEAz{ # } Q<8) u = Z H TRC / A u n/ H Q / A # H Z H 9) Takakura A # TRC rifampin # MGIT cd 100 isoniazid 100 3 " < 3 u#, A # Z H 10) , Ve \ #" 5 H TRC I H PCR y sg - g , & " PCR 2 3 " Sq JK # 2 v L 1 K N # # JK O H P # # O NVe " H " e, H 3 JK # "1,000 ) R { Vol. 18 No. 1 / TRC / 1717 770 1,000 !}

8 Q$ & '( ) *1,000 +, - /0 3*TRC4 6 7 :;(* ?@ABC PCRF H J 7 M*N J TRCF RST U W J T7 4 YZH J [ T\T J Z `a*Nb cd 3 7 4 J T * 3 Re f g T f J ! Q$ & h 4 ij J [ Yl T J *mn +, pqF rs( ( v wx Yyz m {|T\ } a ~ \ ! Q$ & '( ) j J * - T 4 7 3 d? * U '( * T\ U T U Q$ qF PCRF v ( U \J * 1) Tenover, F. C., J. T. Crawford, R. E. Huebner, etal. 1993. The resurgence of tuberculosis : isyour laboratory ready? J. Clin.))))]]

9 Microbiol. 31:767 ) Jensen, P. A., L. A. Lambert, M. F. Guidelines for preventing the transmis-sion ofMycobacterium tuberculosisin health-care settings, 2005. MMWR Recomm. Rep. 54:1 ) Ishiguro, T., J. Saitoh, R. Horie, et al. activating fluorescence DNAprobe and its application to homogeneousquantification of a target sequence by isother-mal sequence amplification in a closed Biochem. 314: 77 ) Ishiguro, T., J. Saitoh, H. Yawata, et al. detection of specific sequence ofnucleic acids by oxazole yellow-linked oligonu-cleotides. Homogeneous quantitative monitor-ing ofin vitrotranscription. Nucleic Acids : 4992 ) Takakura, S.

10 , S. Tsuchiya, Y. Isawa, et al. detection ofMycobacteriumu tuberculosisin respiratory samples by transcription-reversetranscription concerted reaction with an auto-mated system. L. Clin. Microbiol. 43: 5435 ) Nakaguchi, Y., T. Ishizuka, T. Hayashi, et Rapid and specific detection of tdh, trh1,and trh2 mRNA ofVibrio parahaemolyticusbytranscription-reverse transcription concertedreaction with an automated system. Clin. Mi-crobiol. 42: 4284 ) Ishii, T., Y. Fujiwara, S. Ohnaka, et al. genetic diagnosis with the transcrip-tion reverse transcription concerted reactionsystem for cancer micrometastasis. Ann. 11: 778 ) Kaito, K.