Tryptic Soya Agar - himedialabs.com

1 Please refer disclaimer soya AgarM1968 Tryptic soya agar is a general purpose medium used for a cultivation and maintenance of Salmonella Typhi. Composition**IngredientsGms / LitrePancreatic digest of digest of soyabean (Glucose) hydrogen pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend 45 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at15lbs pressure (121 C) for 15 minutes. Cool to 45-50 C. Mix well and pour into sterile Petri And InterpretationTryptic soya agar supports the growth of wide variety of organisms including fastidious and non fastidious such as Neisseria,Listeria, Brucella etc (1).

2 Tryptone soya Broth with added dextrose, sodium chloride and agar is recommended for thecultivation of Salmonella Typhi (2).Pancreatic digest of casein and papaic digest of soyabean meal provides nitrogen, vitamins and minerals. Glucose serves as thecarbohydrate source. Phosphate buffers the media. Sodium chloride maintains the osmotic ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with agar gelColour and Clarity of prepared mediumLight yellow coloured clear to slightly opalescent of w/v aqueous solution at 25 C. pH : responseCultural characteristics was observed after an incubation at 30-35 C for 18-24 hours Cultural ResponseOrganismInoculum(CFU)GrowthRecov eryCultural response50-100luxuriant>=70%Salmonella Typhi ATCC6539 Storage and Shelf LifeStore below 30 C in tightly closed container and the prepared medium at 2 - 8 C.

3 Use before expiry date on the LaboratoriesTechnical DataDisclaimer :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified.

4 Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: Website: B. A., Sahm D. F. and Weissfeld A. S., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc. , Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, : 0 / 2014