WL Differential Agar - HiMedia Leading …

1 Please refer disclaimer Differential AgarM1060WL Differential agar is recommended for selective isolation and enumeration of bacteria encountered in breweries andindustrial **IngredientsGms / LitreCasein enzymic cresol pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclavingat 15 lbs pressure (121 C) for 15 minutes. If desired, to obtain a pH of , add 1% solution of sodium bicarbonate before : Cycloheximide is very toxic. Avoid skin contact or aerosol formation and And InterpretationWL (Wallerstein Laboratory) media are formulated as described by Green and Gray for the examination of materialsencountered in brewing and for industrial fermentations containing mixed flora of yeast and bacteria (1, 2).

2 Bakers yeast countscan be carried out in this medium at a pH By adjusting the pH to , the medium can be used for obtaining counts ofBaker and distillers yeast (3).WL Nutrient and WL Differential Media are used in combination. One plate of WL Nutrient agar and two plates of WLDifferential agar are prepared (3). The WL Nutrient agar plate is incubated aerobically to give a total yeast count while oneWL Differential agar plate gives the count of acetic acid bacteria, Flavobacterium , Proteus and thermophilic bacterialcount when incubated aerobically. The other WL Differential agar Plate is incubated anaerobically for the growth of lacticacid bacteria and Pediococcus.

3 While determining microbial counts using these media, temperature and time of incubationwill vary depending on the nature of material under test. Temperatures of 25 C are employed for brewing materials while 30 Care employed for bakers yeast and alcohol fermentation mash Differential medium contain yeast extract, which serves as a source of trace elements, vitamins and amino acids. Caseinenzymic hydrolysate is used as a source of nitrogen, amino acids and carbon. Dextrose is the source of carbohydrate. Bufferingof the medium is done by monopotassium phosphate. Potassium chloride, calcium chloride and ferric chloride are essentialions that help to maintain the osmotic balance.

4 Magnesium sulphate and manganese sulphate are sources of divalent green is a pH indicator. Yeasts and moulds are inhibited by cycloheximide (actidione).Quality ControlAppearanceLight yellow to light green homogeneous free flowing powderGellingHiMedia LaboratoriesTechnical DataHiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: comparable with agar and Clarity of prepared mediumBluish green coloured clear to slightly opalescent gel forms in Petri of w/v aqueous solution at 25 C. pH : ResponseM1060: Cultural characteristics observed after an incubation for 40-48 hours at 35-37 C for bacteria and at 30 2 C (CFU)GrowthRecoveryCultural ResponseEscherichia coli ATCC2592250-100luxuriant>=50%Lactobacill us fermentumATCC 933850-100good40-50%Proteus mirabilis ATCC2593350-100good40-50%Saccharomyces cerevisiaeATCC 9763>=10 inhibited0%Saccharomyces uvarumATCC 28098>=10 inhibited0%Storage and Shelf LifeStore below 30 C in tightly closed container and the prepared medium at 2 - 8 C.

5 Use before expiry date on the Green S. R. and Gray P. P., 1950, Wallerstein Lab. Commun., 12:432. Green S. R. and Gray P. P., 1950, Wallerstein Lab. Commun., 13:3573. MacFaddin J. F., 1985, Media for Isolation- Cultivation- Identification- Maintenance of Medical Bacteria, ,Williams& Wilkins, Baltimore, : 02 / 2015 Disclaimer :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate.

6 HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.