Transcription of 33 Gradient Appendix - RIT - People
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33 Gradient Appendix - RIT - People
142 Appendix VIII Ultracentrifugation Ultracentrifugation, the characterization of macromolecules by centrifugation at super high speeds, is a foundational technique that has greatly enhanced our understanding of molecular biology. The rate of settling or sedimentation of macromolecules under high centrifugal force is a function of their size and shape. A macromolecule studied in this manner is usually characterized according to its S value (sedimentation coefficient), named after Theodor Svedberg, a Swedish chemist who received the Nobel Prize for his work on colloids and his invention of the ultracentrifuge.
Appendix VII 144 Zonal Sedimentation Zonal gradients separate DNA according to size. Gradients, typically sucrose, are preformed by setting up a mixing chamber that has high and low concentration sucrose solutions in each of two chambers.
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