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Comparison of Sample Preparation Methods for the ...

20 The Open Proteomics Journal, 2010, 3, 20-25 1875-0397/10 2010 Bentham Open Open Access Comparison of Sample Preparation Methods for the Resolution of Metal-Regulated Proteins in Helianthus Annuus by 2-Dimensional Gel-Electrophoresis Chamari Walliwalagedara1, Harry van Keulen2,*, Teresa Cutright3 and Robert Wei1 1 Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, USA 2 Department of Biological, Geological, and Environmental Sciences, Cleveland State University, Cleveland, Ohio 44115.

Use of a phenol extraction method enabled better resolution of proteins compared to either acetone or Trichloroacetic acid (TCA/acetone) precipitation techniques. TCA/acetone precipitation combined with a phenol treat-

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  Protein, Acetone, Precipitation, Of protein, Acetone precipitation

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