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Protocols and tips in protein purification

Protocols and tips in protein purification or How to purify protein in one day Second edition 2018 Department of Molecular Biology & Biotechnology 2 3 Contents I. Introduction 7 II. General sequence of protein purification procedures Preparation of equipment and reagents Preparation and use of stock solutions Chromatography system Preparation of chromatographic columns Preparation of crude extract (cell free extract or soluble proteins fraction) Pre chromatographic steps Chromatographic steps Sequence of operations during IEC and HIC Ion exchange chromatography (IEC) Hydrophobic interaction chromatography (HIC) Gel filtration (SEC)

By having stock solutions you can prepare any buffer for protein purification in seconds. Simply pour required volumes of stock solutions in to a Duran bottle and adjust the volume with ultra pure water to the top mark on the bottle. Worked example: to prepare 1 litre of buffer 50mM tris-HCl pH 8, 100 mM NaCl, 2mM EDTA:

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  Solutions, Tips, Protocol, Purification, Protein, Buffer, Protocols and tips in protein purification

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