General western blot protocol - Abcam
Solutions and reagents Lysis buffers These buffers may be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year. NP-40 buffer ‒ 150 mM NaCl ‒ 1.0% NP-40 (possible to substitute with 0.1% Triton X-100) ‒ 50 mM Tris-HCl, pH 8.0 ‒ Protease inhibitors RIPA buffer (radioimmunoprecipitation assay buffer)
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