QPCR Optimization & Troubleshooting Guide
Introduction Whether you are beginning to develop a QPCR assay, have a QPCR assay you want to optimize, or are getting questionable ... Nanodrop), microfluidics or PAGE. Amplicon Size Ideally, the amplicon should always be between 100bp and 150bp to ensure the QPCR reaction efficiency is as close to 100% as possible. Good QPCR efficiency ...
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