QPCR Optimization & Troubleshooting Guide
Adjust baseline correction (ie from cycles 3-15 to 3-10 or dilute template between 1:100 and 1:1000 and repeat). Amplification plot goes up, down and all around Baseline has been set so when software applies data correction curves are distorted Adjust baseline correction (ie from cycles 3-15 to 3-10 or dilute template between
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